School of Biological Science and Engineering, Tokai University, Minamisawa, Minami-ku, Sapporo 005-8601, Japan.
Graduate School of Science and Engineering, Tokai University, Minamisawa, Minami-ku, Sapporo 005-8601, Japan.
Int J Syst Evol Microbiol. 2014 Jan;64(Pt 1):101-106. doi: 10.1099/ijs.0.055558-0. Epub 2013 Sep 10.
A novel exopolysaccharide-producing bacterium, designated strain SE3(T), was isolated from Pacific Ocean sediment. The strain was Gram-stain-negative, motile, strictly aerobic, oxidase-positive and catalase-positive, and required Na(+) for growth. Its major isoprenoid quinone was ubiquinone-8 (Q-8), and its cellular fatty acid profile mainly consisted of C16 : 1ω7c, C16 : 0 and C18 : 1ω7c. The DNA G+C content was 46.9 mol%. 16S rRNA gene sequence analysis suggested that strain SE3(T) is a member of the genus Pseudoalteromonas. Strain SE3(T) exhibited close phylogenetic affinity to Pseudoalteromonas arabiensis JCM 17292(T) (99.0 % 16S rRNA gene sequence similarity), Pseudoalteromonas lipolytica LMEB 39(T) (98.39 %) and Pseudoalteromonas donghaensis HJ51(T) (97.65 %). The DNA-DNA reassociation values between strain SE3(T) and P. arabiensis JCM 17292(T), P. lipolytica JCM 15903(T) and P. donghaensis LMG 24469(T) were 31, 26 and 44 %, respectively. Owing to the significant differences in phenotypic and chemotaxonomic characteristics, phylogenetic analysis based on 16S rRNA gene sequences and DNA-DNA relatedness data, the new isolate merits classification as a representative of novel species, for which the name Pseudoalteromonas shioyasakiensis is proposed. The type strain is SE3(T) ( = JCM 18891(T) = NCIMB 14852(T)).
一株新的产胞外多糖细菌，命名为 SE3(T) 菌株，从太平洋沉积物中分离得到。该菌株革兰氏阴性，运动，严格需氧，氧化酶阳性，过氧化氢酶阳性，生长需要 Na(+)。其主要的类异戊二烯醌是泛醌-8 (Q-8)，其细胞脂肪酸图谱主要由 C16 : 1ω7c、C16 : 0 和 C18 : 1ω7c 组成。DNA G+C 含量为 46.9 mol%。16S rRNA 基因序列分析表明，菌株 SE3(T) 是拟交替单胞菌属的一个成员。菌株 SE3(T)与 Pseudoalteromonas arabiensis JCM 17292(T)（99.0% 16S rRNA 基因序列相似性）、Pseudoalteromonas lipolytica LMEB 39(T)（98.39%）和 Pseudoalteromonas donghaensis HJ51(T)（97.65%）亲缘关系最密切。菌株 SE3(T)与 P. arabiensis JCM 17292(T)、P. lipolytica JCM 15903(T)和 P. donghaensis LMG 24469(T)的 DNA-DNA 同源性分别为 31%、26%和 44%。由于表型和化学分类特征的显著差异，基于 16S rRNA 基因序列和 DNA-DNA 同源性数据的系统发育分析，新分离株值得分类为代表新种，命名为 Shioyasakiensis 假交替单胞菌。模式株为 SE3(T)（ = JCM 18891(T) = NCIMB 14852(T)）。
