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采用原发性干燥综合征患者来源的单核细胞来源的耐受性树突状细胞抑制体外免疫反应。

In vitro suppression of immune responses using monocyte-derived tolerogenic dendritic cells from patients with primary Sjögren's syndrome.

出版信息

Arthritis Res Ther. 2013;15(5):R114. doi: 10.1186/ar4294.

DOI:10.1186/ar4294
PMID:24025795
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3978468/
Abstract

INTRODUCTION

Therapeutic vaccination with antigen-specific tolerogenic dendritic cells (tolDC) might become a future option of individualized therapy for patients with autoimmune diseases. In this study, we tested the possibility of generating monocyte-derived tolDC from patients with primary Sjögren's syndrome (pSS). We analyzed phenotype, cytokine production and ability to suppress Ro/La-specific immune responses.

METHODS

Monocyte-derived tolDC from patients with pSS were generated in the presence of dexamethasone, vitamin D3 and lipopolysaccharide (DexVD3 DC). The phenotype was analyzed by flow cytometry and the cytokine profile was investigated using a 25-plex Luminex assay and ELISA. The capacity to both stimulate Ro/La-specific T cells and suppress this response was evaluated by autologous mixed lymphocyte reaction (MLR).

RESULTS

DC generated from patients with pSS had a similar phenotype and cytokine profile to those from healthy controls. DexVD3 DC from pSS patients induced little antigen-specific T cell proliferation, but DexVD3 DC-primed lymphocytes successfully suppressed Ro/La-specific T cell responses.

CONCLUSIONS

DexVD3 DC presenting Ro/La antigens might be a promising new therapeutic option for patients with pSS.

摘要

简介

用抗原特异性耐受原性树突细胞（tolDC）进行治疗性疫苗接种可能成为治疗自身免疫性疾病患者的未来个体化治疗选择。在这项研究中，我们测试了从原发性干燥综合征（pSS）患者中生成单核细胞来源的 tolDC 的可能性。我们分析了表型、细胞因子产生和抑制 Ro/La 特异性免疫反应的能力。

方法

在地塞米松、维生素 D3 和脂多糖（DexVD3 DC）存在的情况下，从 pSS 患者中生成单核细胞来源的 tolDC。通过流式细胞术分析表型，并使用 25 plex Luminex 测定法和 ELISA 研究细胞因子谱。通过自体混合淋巴细胞反应（MLR）评估刺激 Ro/La 特异性 T 细胞和抑制该反应的能力。

结果

从 pSS 患者中生成的 DC 具有与健康对照相似的表型和细胞因子谱。来自 pSS 患者的 DexVD3 DC 诱导的抗原特异性 T 细胞增殖很少，但 DexVD3 DC 致敏的淋巴细胞成功抑制了 Ro/La 特异性 T 细胞反应。

结论

呈现 Ro/La 抗原的 DexVD3 DC 可能成为 pSS 患者有前途的新治疗选择。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6214/3978468/078d74f5bef6/ar4294-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6214/3978468/4d550b56fc4a/ar4294-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6214/3978468/fc6a0300c23a/ar4294-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6214/3978468/f145c46eba45/ar4294-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6214/3978468/8c828ce1adc1/ar4294-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6214/3978468/078d74f5bef6/ar4294-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6214/3978468/4d550b56fc4a/ar4294-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6214/3978468/fc6a0300c23a/ar4294-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6214/3978468/f145c46eba45/ar4294-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6214/3978468/8c828ce1adc1/ar4294-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6214/3978468/078d74f5bef6/ar4294-5.jpg

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本文引用的文献

1
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Clin Exp Rheumatol. 2015 Jan-Feb;33(1):56-62. Epub 2014 Dec 22.
2
MRP8 promotes Th17 differentiation via upregulation of IL-6 production by fibroblast-like synoviocytes in rheumatoid arthritis.MRP8 通过上调成纤维样滑膜细胞中 IL-6 的产生促进类风湿关节炎中 Th17 的分化。
Exp Mol Med. 2013 Apr 26;45(4):e20. doi: 10.1038/emm.2013.39.
3
The serum IL-6 profile and Treg/Th17 peripheral cell populations in patients with type 1 diabetes.
训练免疫在自身免疫和自身炎症性疾病中的潜在作用。
Front Immunol. 2018 Feb 20;9:298. doi: 10.3389/fimmu.2018.00298. eCollection 2018.
4
Vitamin D in Autoimmunity: Molecular Mechanisms and Therapeutic Potential.自身免疫中的维生素D：分子机制与治疗潜力
Front Immunol. 2017 Jan 20;7:697. doi: 10.3389/fimmu.2016.00697. eCollection 2016.
5
Generation of Tolerogenic Dendritic Cells and Their Therapeutic Applications.耐受性树突状细胞的生成及其治疗应用。
Immune Netw. 2016 Feb;16(1):52-60. doi: 10.4110/in.2016.16.1.52. Epub 2016 Feb 25.
6
Role of Dendritic Cells in the Induction of Lymphocyte Tolerance.树突状细胞在淋巴细胞耐受诱导中的作用。
Front Immunol. 2015 Oct 20;6:535. doi: 10.3389/fimmu.2015.00535. eCollection 2015.
7
Contribution of dendritic cells to the autoimmune pathology of systemic lupus erythematosus.树突状细胞在系统性红斑狼疮自身免疫病理中的作用。
Immunology. 2015 Dec;146(4):497-507. doi: 10.1111/imm.12504. Epub 2015 Oct 12.
8
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9
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1 型糖尿病患者的血清 IL-6 谱和 Treg/Th17 外周细胞群。
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4
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5
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Expert Opin Pharmacother. 2013 Feb;14(3):279-89. doi: 10.1517/14656566.2013.767333. Epub 2013 Jan 25.
6
Targeting interleukin-6: all the way to treat autoimmune and inflammatory diseases.靶向白细胞介素 6：治疗自身免疫和炎症性疾病的新途径。
Int J Biol Sci. 2012;8(9):1227-36. doi: 10.7150/ijbs.4666. Epub 2012 Oct 24.
7
Semi-quantitative evaluation of salivary gland function in Sjögren's syndrome using salivary gland scintigraphy.使用唾液腺闪烁显像术对半干燥综合征唾液腺功能进行半定量评估。
Clin Rheumatol. 2012 Dec;31(12):1699-705. doi: 10.1007/s10067-012-2076-3. Epub 2012 Sep 2.
8
Anti-Ro and anti-La autoantibody profiling in Norwegian patients with primary Sjögren's syndrome using luciferase immunoprecipitation systems (LIPS).采用荧光素酶免疫沉淀系统（LIPS）对挪威原发性干燥综合征患者进行抗Ro和抗La自身抗体分析。
Scand J Rheumatol. 2012 Aug;41(4):314-5. doi: 10.3109/03009742.2012.670863.
9
The cellular and molecular mechanisms of immuno-suppression by human type 1 regulatory T cells.人类 1 型调节性 T 细胞免疫抑制的细胞和分子机制。
Front Immunol. 2012 Feb 29;3:30. doi: 10.3389/fimmu.2012.00030. eCollection 2012.
10
Stable antigen-specific T-cell hyporesponsiveness induced by tolerogenic dendritic cells from multiple sclerosis patients.多发性硬化症患者来源的耐受原性树突状细胞诱导稳定的抗原特异性 T 细胞低反应性。
Eur J Immunol. 2012 Mar;42(3):771-82. doi: 10.1002/eji.201141835.