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[生物滴滤池去除苯系物及相应细菌群落分析]

[Removal of BTEX by a biotrickling filter and analysis of corresponding bacterial communities].

作者信息

Li Jian-Jun, Liao Dong-Qi, Xu Mei-Ying, Sun Guo-Ping

机构信息

Guangdong Institute of Microbiology, Guangzhou 510070, China.

出版信息

Huan Jing Ke Xue. 2013 Jul;34(7):2552-9.

Abstract

The pre-acclimated microbial consortium and the activated sludge were used as start inoculums of a bench-scale biotrickling filter (BTF). The performance of the biotrickling filter on the removal of BTEX mixture was evaluated, and the changes in the bacterial community structure of the BTF were analyzed by PCR-DGGE technique. The results showed that the BTF could be acclimated within a short time, the biomass that adhered to the surface of packing materials increased rapidly from 5.7 mg x g(-1) at 10th day to 112 mg x g(-1) at 30th day. BTF could simultaneously remove all components of the BTEX mixture efficiently. The maximum removal capacity of the BTF was 216.6 g x (m3 x h)(-1), which was achieved with an inlet loading rate of 269.7 g x (m3 x h)(-1) and an empty bed retention time (EBRT) of 39 s. DGGE analysis indicated that the dominant microorganisms may be derived from the pre-acclimated microbial consortiums rather than the activated sludge. Although the bacterial community changed with run time, the spatial distribution was very uniform.

摘要

将预驯化的微生物群落和活性污泥用作实验室规模生物滴滤池(BTF)的起始接种物。评估了生物滴滤池对BTEX混合物的去除性能,并采用PCR-DGGE技术分析了生物滴滤池中细菌群落结构的变化。结果表明,生物滴滤池可在短时间内完成驯化,附着在填料表面的生物量从第10天的5.7 mg x g(-1)迅速增加到第30天的112 mg x g(-1)。生物滴滤池能够高效地同时去除BTEX混合物的所有组分。生物滴滤池的最大去除能力为216.6 g x (m3 x h)(-1),这是在入口负荷率为269.7 g x (m3 x h)(-1)且空床停留时间(EBRT)为39 s的条件下实现的。DGGE分析表明,优势微生物可能源自预驯化的微生物群落而非活性污泥。尽管细菌群落随运行时间发生变化,但其空间分布非常均匀。

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