Zhang Yuanyuan, Atala Anthony
Department of Urology, Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, NC, USA.
Methods Mol Biol. 2013;1037:27-43. doi: 10.1007/978-1-62703-505-7_2.
This chapter reviews the use of urothelial cells as a means to enhance tissue regeneration and wound healing in urinary tract system. It addresses the properties of urothelial cells, including their role as a permeability barrier to protect underlying muscle tissue from the caustic effects of urine and as one of the main cell types, along with smooth muscle cells, that are used in urethral or bladder tissue engineering today. This description includes a general overview of various isolation techniques and culture methods that have been developed to improve urinary tract reconstruction in vivo and aid the characterization of growth factor expression in vitro. The chapter then describes various applications using urothelial cells, including production of multilayer urothelial sheets, tissue engineered bladder mucosa, tissue engineered urethra, and tissue engineered bladder. It also outlines the advantages of sandwich and layered coculture of these cells and the effects of epithelial-stromal cell interactions during tissue regeneration or wound healing processes in the urinary tract.
本章回顾了使用尿路上皮细胞作为增强泌尿系统组织再生和伤口愈合的一种手段。它阐述了尿路上皮细胞的特性，包括其作为渗透屏障保护下层肌肉组织免受尿液腐蚀性影响的作用，以及作为当今尿道或膀胱组织工程中使用的主要细胞类型之一，与平滑肌细胞一起的作用。这一描述包括对各种已开发的分离技术和培养方法的概述，这些技术和方法旨在改善体内尿路重建并有助于体外生长因子表达的表征。本章接着描述了使用尿路上皮细胞的各种应用，包括多层尿路上皮片的生产、组织工程膀胱黏膜、组织工程尿道和组织工程膀胱。它还概述了这些细胞的三明治式和分层共培养的优点，以及尿路组织再生或伤口愈合过程中上皮 - 基质细胞相互作用的影响。
