Kanagawa R, Saika S, Miyamoto K, Ohmi S, Tamura M, Nakao T
Department of Ophthalmology, Wakayama Medical College, Japan.
Nippon Ganka Gakkai Zasshi. 1990 Jun;94(6):549-52.
An immunohistochemical study was performed to observe cellular proliferation on the surface of implanted intraocular lenses (IOLs) in rabbit eyes. Rabbits were killed 3-28 days postoperatively. The removed IOLs were examined by an immunoperoxidase staining method using anti-fibronectin antibodies. The immunoreactivity for fibronectin was detected in macrophages and multinucleated giant cells attached to the IOL surface. Prominent stains were observed in these cells 1 week after the operation, and staining for fibronectin was less intense in the specimens 2 and 4 weeks after implantation. Fibronectin is produced by macrophages and multinucleated giant cells on the IOL surface, and might play important role in cellular adhesion and motility. Fibronectin immunoreactivity decreased with time and it may be related to cellular activity.
进行了一项免疫组织化学研究，以观察兔眼植入人工晶状体（IOL）表面的细胞增殖情况。术后3至28天处死兔子。使用抗纤连蛋白抗体，通过免疫过氧化物酶染色法检查取出的人工晶状体。在附着于人工晶状体表面的巨噬细胞和多核巨细胞中检测到纤连蛋白的免疫反应性。术后1周在这些细胞中观察到明显的染色，而在植入后2周和4周的标本中，纤连蛋白的染色较弱。纤连蛋白由人工晶状体表面的巨噬细胞和多核巨细胞产生，可能在细胞黏附和运动中起重要作用。纤连蛋白免疫反应性随时间降低，这可能与细胞活性有关。
