Kanagawa R, Saika S, Ohmi S, Tamura M, Nakao T
Department of Ophthalmology, Wakayama Medical College, Japan.
Graefes Arch Clin Exp Ophthalmol. 1990;228(5):398-400. doi: 10.1007/BF00927249.
An immunohistochemical study was performed to observe cellular proliferation on the surface of implanted intraocular lenses (IOLs) in rabbit eyes. Rabbits were killed at intervals of 3 days and 1, 2, and 4 weeks after the operation. The IOLs were removed and examined by an immunoperoxidase staining method using antifibronectin (anti-FN) antibodies. The FN immunoreactivity was detected in macrophages and giant cells attached to the IOL surface. Prominent staining was observed in these cells 1 week after the operation, and staining for FN was less intense in the specimens obtained 2 and 4 weeks after implantation. These findings suggest that FN is produced by macrophages and giant cells on the IOL surface and may play an important role in cellular adhesion and motility. FN immunoreactivity decreased with time, which might be related to cellular activity.
进行了一项免疫组织化学研究，以观察兔眼内植入人工晶状体（IOL）表面的细胞增殖情况。术后每隔3天以及1、2和4周处死兔子。取出IOL，使用抗纤连蛋白（anti-FN）抗体通过免疫过氧化物酶染色法进行检查。在附着于IOL表面的巨噬细胞和巨细胞中检测到FN免疫反应性。术后1周在这些细胞中观察到明显的染色，而在植入后2周和4周获得的标本中FN染色强度较弱。这些发现表明FN由IOL表面的巨噬细胞和巨细胞产生，可能在细胞黏附和运动中起重要作用。FN免疫反应性随时间降低，这可能与细胞活性有关。
