Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK.
Science. 2013 Sep 27;341(6153):1514-7. doi: 10.1126/science.1240578. Epub 2013 Sep 12.
The global epidemic of multidrug-resistant Salmonella Typhimurium DT104 provides an important example, both in terms of the agent and its resistance, of a widely disseminated zoonotic pathogen. Here, with an unprecedented national collection of isolates collected contemporaneously from humans and animals and including a sample of internationally derived isolates, we have used whole-genome sequencing to dissect the phylogenetic associations of the bacterium and its antimicrobial resistance genes through the course of an epidemic. Contrary to current tenets supporting a single homogeneous epidemic, we demonstrate that the bacterium and its resistance genes were largely maintained within animal and human populations separately and that there was limited transmission, in either direction. We also show considerable variation in the resistance profiles, in contrast to the largely stable bacterial core genome, which emphasizes the critical importance of integrated genotypic data sets in understanding the ecology of bacterial zoonoses and antimicrobial resistance.
全球范围内耐多药鼠伤寒沙门氏菌 DT104 的流行提供了一个重要的例子，无论是在病原体及其耐药性方面，都是一种广泛传播的人畜共患病原体。在这里，我们利用前所未有的全国范围内同时从人类和动物中收集的分离株的集合，包括国际衍生分离株的样本，通过一次流行，使用全基因组测序来剖析细菌及其抗微生物耐药基因的系统发育关联。与当前支持单一同质流行的原则相反，我们证明了细菌及其耐药基因在动物和人类群体中基本上是分开维持的，并且在任何方向上的传播都很有限。我们还显示了耐药谱的相当大的差异，与基本上稳定的细菌核心基因组形成对比，这强调了综合基因型数据集在理解细菌人畜共患病和抗微生物耐药性的生态学方面的重要性。
