Department of Pharmaceutical Sciences, Università del Piemonte Orientale "A. Avogadro", Largo Donegani 2, 28100, Novara, Italy.
Neurol Sci. 2014 Mar;35(3):421-7. doi: 10.1007/s10072-013-1535-1. Epub 2013 Sep 13.
The present study was designed to replicate previous findings reporting a significant association between the rs548294 polymorphism at the glutamate receptor subunit GluR1 gene (GRIA1) and migraine without aura, either as a single marker or in haplotype combination with rs2195450. In addition, the role of GRIA1 polymorphisms and haplotypes was evaluated in migraine patients without aura as predictive factors for consistency in headache response to triptans. Analysis of rs548294 and rs2195450 polymorphisms of GRIA1 was conducted by Real-time PCR allelic discrimination assay in 186 migraine patients without aura and 312 healthy controls, respectively. In the logistic regression analysis adjusted for gender and age, genotype and haplotype frequencies for the two polymorphisms did not significantly differ between migraine patients without aura and controls. In addition, no evidence of association was found between GRIA1 polymorphisms/haplotypes and consistent response to triptans. This study failed to replicate previously reported association between GRIA1 rs548294 and migraine without aura, either as single marker or when analyzed in haplotype combination with rs2195450. In addition, no evidence was found for a relevant role of GRIA1 polymorphisms and haplotypes as modulating factors of headache response to triptans.
本研究旨在复制先前的研究结果，这些研究报告谷氨酸受体亚基 GluR1 基因（GRIA1）的 rs548294 多态性与无先兆偏头痛之间存在显著关联，无论是作为单一标志物还是与 rs2195450 形成单体型组合。此外，还评估了 GRIA1 多态性和单体型在无先兆偏头痛患者中的作用，作为曲普坦类药物对头痛反应一致性的预测因子。通过实时 PCR 等位基因鉴别分析，分别在 186 例无先兆偏头痛患者和 312 例健康对照者中分析了 GRIA1 的 rs548294 和 rs2195450 多态性。在调整性别和年龄的逻辑回归分析中，两种多态性的基因型和单体型频率在无先兆偏头痛患者和对照组之间无显著差异。此外，在 GRIA1 多态性/单体型与曲普坦类药物反应一致性之间也未发现关联。本研究未能复制先前报道的 GRIA1 rs548294 与无先兆偏头痛之间的关联，无论是作为单一标志物还是与 rs2195450 分析单体型组合时均如此。此外，也没有证据表明 GRIA1 多态性和单体型作为曲普坦类药物对头痛反应的调节因子具有相关性。
