受碱性磷酸酶活性限制的大肠杆菌K-12恒化器培养
Chemostat culture of Escherichia coli K-12 limited by the activity of alkaline phosphatase.
作者信息
King S L, Francis J C
出版信息
Appl Microbiol. 1975 Aug;30(2):267-70. doi: 10.1128/am.30.2.267-270.1975.
Abstract
The growth-limiting reaction of a chemostat culture of Escherichia coli K-12 was the hydrolysis of beta-glycerophosphate by alkaline phosphatase. The culture was buffered at pH 5.2 where alkaline phosphatase was unable to supply phosphate to the cell at a rate sufficient to sustain the maximum rate of growth. Alkaline phosphatase activity in this system is discussed in terms of the so-called Flip-Flop mechanism.
摘要
大肠杆菌K-12恒化器培养物的生长限制反应是碱性磷酸酶催化β-甘油磷酸的水解。培养物在pH 5.2下缓冲，在此条件下碱性磷酸酶无法以足以维持最大生长速率的速度向细胞供应磷酸盐。该系统中的碱性磷酸酶活性根据所谓的翻转机制进行了讨论。
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