Programa de Pós-graduação em Ciências Veterinária, Faculdade Veterinária, Universidade Federal do Rio Grande do Sul , Porto Alegre, RS , Brasil.
Braz J Microbiol. 2010 Jan;41(1):116-23. doi: 10.1590/S1517-838220100001000018. Epub 2010 Mar 1.
The aim of this study was to evaluate the in vitro activity of the essential oil extracted from Origanum vulgare against sixteen Candida species isolates. Standard strains tested comprised C. albicans (ATCC strains 44858, 4053, 18804 and 3691), C. parapsilosis (ATCC 22019), C. krusei (ATCC 34135), C. lusitaniae (ATCC 34449) and C. dubliniensis (ATCC MY646). Six Candida albicans isolates from the vaginal mucous membrane of female dogs, one isolate from the cutaneous tegument of a dog and one isolate of a capuchin monkey were tested in parallel. A broth microdilution technique (CLSI) was used, and the inoculum concentration was adjusted to 5 x 10(6) CFU mL(-1). The essential oil was obtained by hydrodistillation in a Clevenger apparatus and analyzed by gas chromatography. Susceptibility was expressed as Minimal Inhibitory Concentration (MIC) and Minimal Fungicidal Concentration (MFC). All isolates tested in vitro were sensitive to O. vulgare essential oil. The chromatographic analysis revealed that the main compounds present in the essential oil were 4-terpineol (47.95%), carvacrol (9.42%), thymol (8.42%) and □-terpineol (7.57%). C. albicans isolates obtained from animal mucous membranes exhibited MIC and MFC values of 2.72 μL mL(-1) and 5 μL mL(-1), respectively. MIC and MFC values for C. albicans standard strains were 2.97 μL mL(-1) and 3.54 μL mL(-1), respectively. The MIC and MFC for non-albicans species were 2.10 μL mL(-1) and 2.97 μL mL(-1), respectively. The antifungal activity of O. vulgare essential oil against Candida spp. observed in vitro suggests its administration may represent an alternative treatment for candidiasis.
本研究旨在评估牛至精油对 16 株念珠菌分离株的体外活性。测试的标准菌株包括白色念珠菌（ATCC 菌株 44858、4053、18804 和 3691）、近平滑念珠菌（ATCC 22019）、克柔念珠菌（ATCC 34135）、乳酒念珠菌（ATCC 34449）和都柏林念珠菌（ATCC MY646）。同时还测试了来自雌性犬阴道黏膜的 6 株白色念珠菌分离株、1 株来自犬皮肤表皮的分离株和 1 株来自卷尾猴的分离株。采用肉汤微量稀释技术（CLSI），将接种浓度调整为 5 x 10(6) CFU mL(-1)。精油通过水蒸汽蒸馏在克利夫兰装置中获得，并通过气相色谱进行分析。敏感性表示为最小抑菌浓度（MIC）和最小杀菌浓度（MFC）。体外测试的所有分离株均对牛至精油敏感。色谱分析表明，精油中的主要化合物为 4-松油醇（47.95%）、香芹酚（9.42%）、百里酚（8.42%）和 □-松油醇（7.57%）。从动物黏膜获得的白色念珠菌分离株的 MIC 和 MFC 值分别为 2.72 μL mL(-1)和 5 μL mL(-1)。白色念珠菌标准菌株的 MIC 和 MFC 值分别为 2.97 μL mL(-1)和 3.54 μL mL(-1)。非白色念珠菌的 MIC 和 MFC 值分别为 2.10 μL mL(-1)和 2.97 μL mL(-1)。牛至精油对念珠菌属的体外抗真菌活性表明，其给药可能是治疗念珠菌病的一种替代方法。
