Naeini A, Naderi N Jalayer, Shokri H
Traditional Medicine Clinical Trail Research Center, Shahed University, Tehran, Iran.
Oral and Maxillofacial Pathology Department, Faculty of Dentistry, Shahed University, Tehran, Iran.
J Mycol Med. 2014 Mar;24(1):13-8. doi: 10.1016/j.mycmed.2013.09.006. Epub 2013 Nov 8.
The in vitro antifungal activities of essential oil from Cuminum cyminum (C. cyminum) and alcoholic extract from Salvadora persica (S. persica) were investigated in order to evaluate their efficacy against C. albicans ATCC 14053, C. dubliniensis ATCC CD60, C. glabrata ATCC 90030, C. krusei ATCC 6258 and C. parapsilosis ATCC 22019.
The essential oil was obtained by hydrodistillation in a Clevenger apparatus and analyzed by gas chromatography/mass spectroscopy (GC/MS). The disc diffusion and broth macrodilution methods were used as antifungal susceptibility tests.
The GC/MS analysis allowed 17 components to be determined; the main constituents of C. cyminum essential oil were α-pinene (30%), limonene (21%) and 1,8-cineole (18.5%). C. cyminum oil had a broad-spectrum antifungal activity against different pathogenic Candida species. Inhibition zone values ranged from 7 to 50mm for C. cyminum and 0 to 10mm for S. persica against the organisms tested. The best minimal inhibitory concentration (MIC) of C. cyminum oil was associated with C. albicans and C. dubliniensis (289 mg/L) and the MICs of S. persica extract were 4.9 mg/mL and 20mg/mL against C. albicans and C. dubliniensis, respectively.
The results suggested the potential substitution of the antifungal chemicals by C. cyminum essential oil and S. persica alcoholic extract as natural inhibitors to control the growth of the most important pathogenic Candida species and alternative therapies for candidiasis.
研究孜然（Cuminum cyminum）精油和没食子（Salvadora persica）乙醇提取物的体外抗真菌活性，以评估它们对白色念珠菌ATCC 14053、都柏林念珠菌ATCC CD60、光滑念珠菌ATCC 90030、克柔念珠菌ATCC 6258和近平滑念珠菌ATCC 22019的疗效。
通过在克利夫兰装置中进行水蒸馏获得精油，并通过气相色谱/质谱（GC/MS）进行分析。采用纸片扩散法和肉汤稀释法作为抗真菌药敏试验。
GC/MS分析确定了17种成分；孜然精油的主要成分是α-蒎烯（30%）、柠檬烯（21%）和1,8-桉叶素（18.5%）。孜然油对不同致病性念珠菌具有广谱抗真菌活性。孜然对受试菌株的抑菌圈值为7至50毫米，没食子为0至10毫米。孜然油对白色念珠菌和都柏林念珠菌的最低抑菌浓度（MIC）最佳（289毫克/升），没食子提取物对白色念珠菌和都柏林念珠菌的MIC分别为4.9毫克/毫升和20毫克/毫升。
结果表明，孜然精油和没食子乙醇提取物有可能替代抗真菌化学药物，作为天然抑制剂来控制最重要的致病性念珠菌的生长，并作为念珠菌病的替代疗法。
