Departamento de Clínicas Veterinárias, Universidade Estadual de Londrina , Londrina, PR , Brasil.
Braz J Microbiol. 2011 Jan;42(1):290-7. doi: 10.1590/S1517-83822011000100037.
Ten captive neotropical Brazilian feline were submitted to gastroscopic examination and samples of gastric mucosa from fundus, corpus and pyloric antrum were evaluated for the presence of Helicobacter species. Warthin-Starry (WS) staining and PCR assay with species-specific primers and enzymatic cleavage were applied for bacterial detection and identification. Histological lesions were evaluated by haematoxylin and eosin staining. All animals showed normal gross aspect of gastric mucosa. Helicobacter heilmannii was confirmed in 100% of the samples by WS and PCR assay. Mild lymphocytic infiltrate in the lamina propria was observed in eight animals, mainly in the fundus region. Small lymphoid follicles were seen in three animals. No significant association between Helicobacter infection and histological findings was verified. These observations suggest that gastric Helicobacter spp. could be a commensal or a eventual pathogen to captive neotropical feline, and that procedures, way life, and stress level on the shelter apparently had no negative repercussion over the integrity of the stomach.
十只圈养的新热带地区巴西猫接受了胃镜检查,从胃底、胃体和幽门窦采集胃黏膜样本,评估是否存在幽门螺杆菌种属。采用沃辛-斯塔里(Warthin-Starry,WS)染色和种特异性引物的 PCR 检测及酶切法进行细菌检测和鉴定。通过苏木精-伊红(hematoxylin and eosin,HE)染色评估组织学病变。所有动物的胃黏膜大体外观均正常。WS 和 PCR 检测均证实 100%的样本中存在幽门螺旋杆菌。8 只动物的固有层有轻度淋巴细胞浸润,主要在胃底区域。3 只动物可见小的淋巴滤泡。未发现幽门螺杆菌感染与组织学发现之间存在显著相关性。这些观察结果表明,胃幽门螺杆菌可能是圈养新热带地区猫的共生菌或潜在病原体,收容所的程序、生活方式和应激水平对胃的完整性显然没有负面影响。