Happonen I, Saari S, Castren L, Tyni O, Hänninen M L, Westermarck E
Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Finland.
Zentralbl Veterinarmed A. 1996 Jul;43(5):305-15. doi: 10.1111/j.1439-0442.1996.tb00457.x.
The occurrence and topographical mapping of the gastric Helicobacter-like organisms (GHLOs) and their association with histological changes were studied in apparently healthy dogs and cats. Multiple samples were collected for histological examination from the fundus, corpus and antrum of the stomach of 10 dogs and 10 cats. Fundus and corpus were also sampled for transmission electron microscopy (three dogs, six cats), and for culture (eight dogs, six cats). In all dogs, GHLOs were detected in the fundus and corpus, and in the antrum of nine dogs, and significantly more often in the fundus and corpus (in all sample sites examined) than the antrum (P < 0.01). In cats, GHLOs were demonstrated in 6/10 individuals, and in all regions and sample sites. In dogs GHLOs were detected in all sample sites of the fundus and corpus. Lymphocytes, plasma cells and lymphocyte aggregates were found in all dogs in all regions; there were significantly more plasma cells in the antrum than in the corpus (P < 0.05). Neutrophils were found in six dogs, and eosinophils in seven dogs. In cats, lymphocyte aggregates were found only in GHLO-positive cats, which also had more lymphocytes in the fundus and corpus than GHLO-negative ones (P < 0.5). In dogs, no statistically significant association was found between the number of GHLOs and inflammatory parameters. Four dogs showed histological changes comparable to mild chronic gastritis and another six dogs to mild active chronic gastritis. Mild chronic gastritis was found in the antrum of all cats, and it occurred significantly more often in the antrum than in other regions (P < 0.01). In cats, there was a statistically significant association between GHLOs and chronic gastritis in the fundus and corpus (P < 0.05). GHLOs resembling human 'Helicobacter heilmannii' were identified in all the dogs and cats studied by electron microscopy, and Helicobacter felis in one dog in addition. Culture was successful in three dogs and one cat; 'H, heilmannii' was identified in two of the dogs, and H. felis in the third dog and the cat. GHLOs were found to be common in apparently health dogs and cats. Based on the results of this study, one sample from the fundus and corpus is enough to demonstrate GHLOs. In cats, GHLOs may cause histological changes comparable to chronic gastritis, but in dogs this association remain unclear. It is also questionable if the histological criteria for human gastritis, used in the present study, are suitable for dogs and cats.
对看似健康的犬猫，研究了胃内类幽门螺杆菌（GHLOs）的发生、定位及其与组织学变化的关系。从10只犬和10只猫的胃底、胃体和胃窦采集多个样本进行组织学检查。还从胃底和胃体采集样本用于透射电子显微镜检查（3只犬、6只猫）和培养（8只犬、6只猫）。在所有犬中，胃底和胃体均检测到GHLOs，9只犬的胃窦也检测到，且在胃底和胃体（所有检查的样本部位）中检测到的频率显著高于胃窦（P<0.01）。在猫中，10只中有6只检测到GHLOs，且在所有区域和样本部位均有发现。在犬中，胃底和胃体的所有样本部位均检测到GHLOs。在所有犬的所有区域均发现淋巴细胞、浆细胞和淋巴细胞聚集；胃窦中的浆细胞显著多于胃体（P<0.05）。6只犬中发现中性粒细胞，7只犬中发现嗜酸性粒细胞。在猫中，仅在GHLO阳性的猫中发现淋巴细胞聚集，且胃底和胃体中的淋巴细胞也比GHLO阴性的猫多（P<0.5）。在犬中，未发现GHLOs数量与炎症参数之间有统计学意义的关联。4只犬表现出与轻度慢性胃炎相当的组织学变化，另外6只犬表现为轻度活动性慢性胃炎。所有猫的胃窦均发现轻度慢性胃炎，且胃窦中出现的频率显著高于其他区域（P<0.01）。在猫中，GHLOs与胃底和胃体的慢性胃炎之间存在统计学意义的关联（P<0.05）。通过电子显微镜在所有研究的犬猫中均鉴定出类似人类“海尔曼螺杆菌”的GHLOs，另外在1只犬中鉴定出猫螺杆菌。3只犬和1只猫培养成功；2只犬中鉴定出“海尔曼螺杆菌”，第3只犬和猫中鉴定出猫螺杆菌。发现GHLOs在看似健康的犬猫中很常见。根据本研究结果，从胃底和胃体采集一个样本就足以证明GHLOs的存在。在猫中，GHLOs可能引起与慢性胃炎相当的组织学变化，但在犬中这种关联尚不清楚。本研究中使用的人类胃炎组织学标准是否适用于犬猫也值得怀疑。
