Key Laboratory of Biometallurgy of Ministry of Education of China, School of Minerals Processing and Bioengineering , Central South University, Changsha, Hunan 410083 , China ; Biological Resources Institute, Jiangxi Academy of Sciences , Nanchang, Jiangxi 330029 , China.
Braz J Microbiol. 2011 Apr;42(2):514-25. doi: 10.1590/S1517-838220110002000016. Epub 2011 Jun 1.
A novel thermoacidophilic iron and sulfur-oxidizing archaeon, strain YN25, was isolated from an in situ enriched acid hot spring sample collected in Yunnan, China. Cells were irregular cocci, about 0.9-1.02 µm × 1.0-1.31 µm in the medium containing elemental sulfur and 1.5-2.22 µm × 1.8-2.54 µm in ferrous sulfate medium. The ranges of growth and pH were 50-85 (optimum 65) and pH 1.0-6.0 (optimum 1.5-2.5). The acidophile was able to grow heterotrophically on several organic substrates, including various monosaccharides, alcohols and amino acids, though the growth on single substrate required yeast extract as growth factor. Growth occurred under aerobic conditions or via anaerobic respiration using elemental sulfur as terminal electron acceptor. Results of morphology, physiology, fatty acid analysis and analysis based on 16S rRNA gene sequence indicated that the strain YN25 should be grouped in the species Acidianus manzaensis. Bioleaching experiments indicated that this strain had excellent leaching capacity, with a copper yielding ratio up to 79.16% in 24 d. The type strain YN25 was deposited in China Center for Type Culture Collection (=CCTCCZNDX0050).
一株新型嗜酸耐热铁硫氧化古菌 YN25，从中国云南原位富集酸热泉样品中分离得到。细胞呈不规则球菌，在含元素硫的培养基中约为 0.9-1.02 µm×1.0-1.31 µm，在硫酸亚铁培养基中约为 1.5-2.22 µm×1.8-2.54 µm。生长和 pH 值范围为 50-85（最佳 65）和 1.0-6.0（最佳 1.5-2.5）。该嗜酸菌能够异养生长在几种有机底物上，包括各种单糖、醇和氨基酸，但在单一底物上的生长需要酵母提取物作为生长因子。在好氧条件下或通过以元素硫为末端电子受体的无氧呼吸进行生长。形态学、生理学、脂肪酸分析和基于 16S rRNA 基因序列的分析结果表明，菌株 YN25 应归属于嗜酸热硫化叶菌属。生物浸出实验表明，该菌株具有出色的浸出能力，在 24 天内铜的浸出率高达 79.16%。模式菌株 YN25 已保藏在中国典型培养物保藏中心（=CCTCCZNDX0050）。
