Xiang Xiaoyu, Dong Xiuzhu, Huang Li
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
Extremophiles. 2003 Dec;7(6):493-8. doi: 10.1007/s00792-003-0355-2. Epub 2003 Sep 2.
A novel thermoacidophilic strain, designated RT8-4, was isolated from an acidic hot spring in Tengchong, Yunnan, China, and characterized phenotypically and phylogenetically. Cells of strain RT8-4 are irregular cocci with peritrechous flagella. The strain grows aerobically in either a lithotrophic or a heterotrophic mode. No anaerobic growth is apparent. Growth on elemental sulfur occurs through the oxidation of sulfur. Strain RT8-4 is capable of utilizing tryptone, d-xylose, d-arabinose, d-galactose, maltose, sucrose, d-fructose, or l-glutamic acid as the sole source of carbon. d-Glucose and d-mannose are not utilized. RT8-4 grows optimally at 85 degrees C and pH 3.5. The G+C content of the genome of RT8-4 is 34.4 mol%. Phylogenetic analysis based on 16S rDNA sequence as well as DNA-DNA hybridization and phenotypic characterization identifies strain RT8-4 as a novel species in the genus Sulfolobus. It is proposed that strain RT8-4 be designated as Sulfolobus tengchongensis sp. nov. The type strain is RT8-4(T).
从中国云南腾冲的一个酸性温泉中分离出一株新型嗜热嗜酸菌株，命名为RT8-4，并对其进行了表型和系统发育特征分析。RT8-4菌株的细胞为不规则球菌，具周生鞭毛。该菌株能以自养或异养模式进行有氧生长。未见厌氧生长。在单质硫上生长是通过硫的氧化实现的。RT8-4菌株能够利用胰蛋白胨、d-木糖、d-阿拉伯糖、d-半乳糖、麦芽糖、蔗糖、d-果糖或l-谷氨酸作为唯一碳源。不利用d-葡萄糖和d-甘露糖。RT8-4在85℃和pH 3.5条件下生长最佳。RT8-4基因组的G+C含量为34.4 mol%。基于16S rDNA序列、DNA-DNA杂交和表型特征的系统发育分析确定RT8-4菌株为硫化叶菌属的一个新物种。建议将RT8-4菌株命名为腾冲硫化叶菌（Sulfolobus tengchongensis）新种。模式菌株为RT8-4(T)。
