Romanian Polar Research Institute , Bucharest , Romania.
Braz J Microbiol. 2011 Jul;42(3):868-77. doi: 10.1590/S1517-83822011000300005. Epub 2011 Sep 1.
The aim of this study was to isolate novel enzyme-producing bacteria from vegetation samples from East Antarctica and also to characterize them genetically and biochemically in order to establish their phylogeny. The ability to grow at low temperature and to produce amylases and proteases cold-active was also tested. The results of the 16S rRNA gene sequence analysis showed that the 4 Alga rRNA was 100% identical to the sequences of Streptomyces sp. rRNA from Norway and from the Solomon Islands. The Streptomyces grew well in submerged system at 20°C, cells multiplication up to stationary phase being drastically increased after 120 h of submerged cultivation. The beta-amylase production reached a maximum peak after seven days, while alpha-amylase and proteases were performing biosynthesis after nine days of submerged cultivation at 20°C. Newly Streptomyces were able to produce amylase and proteases in a cold environment. The ability to adapt to low temperature of these enzymes could make them valuable ingredients for detergents, the food industry and bioremediation processes which require low temperatures.
本研究的目的是从南极洲东部的植被样本中分离出具有产酶能力的新型细菌，并对其进行遗传和生化特征分析，以确定其系统发育关系。同时，还测试了它们在低温下生长、产生淀粉酶和蛋白酶的能力。16S rRNA 基因序列分析结果表明，4 株藻 rRNA 与来自挪威和所罗门群岛的链霉菌 rRNA 的序列完全一致。链霉菌在 20°C 下的浸没式培养系统中生长良好，经过 120 小时的浸没培养后，细胞倍增达到静止期时，数量急剧增加。β-淀粉酶在七天后达到最大峰值，而α-淀粉酶和蛋白酶在 20°C 下的浸没培养九天后开始进行生物合成。新型链霉菌能够在低温环境下产生淀粉酶和蛋白酶。这些酶适应低温的能力使它们成为洗涤剂、食品工业和生物修复过程的有价值的成分，这些过程需要低温。
