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香菇木聚糖酶的热稳定性。

Thermostability of xylanolytic enzymes produced by Lentinula edodes UFV70.

机构信息

Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto , Ribeirão Preto, SP , Brasil.

出版信息

Braz J Microbiol. 2012 Jan;43(1):201-4. doi: 10.1590/S1517-838220120001000021. Epub 2012 Jun 1.

DOI:10.1590/S1517-838220120001000021
PMID:24031818
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3768989/
Abstract

Xylanolytic enzymes produced by Lentinula edodes UFV70, cultivated in eucalyptus sawdust/rice bran medium, were stable at 50, 60 and 65°C for 21 hours, losing only 15-25% activity. Fungus incubation at 50°C for 12 hours and at 65°C for 24 hours increased the amount of xylose produced.

摘要

巴西蘑菇 UFV70 在以桉木屑/米糠为基质培养时所产生的木聚糖酶，在 50°C、60°C 和 65°C 下稳定 21 小时，活力仅丧失 15-25%。真菌在 50°C 下培养 12 小时和在 65°C 下培养 24 小时，可提高木糖的产量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/648e/3768989/0c79be2f3e23/bjm-43-201-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/648e/3768989/267da1cee0b0/bjm-43-201-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/648e/3768989/0c79be2f3e23/bjm-43-201-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/648e/3768989/267da1cee0b0/bjm-43-201-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/648e/3768989/0c79be2f3e23/bjm-43-201-g002.jpg

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本文引用的文献

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Lignocellulosic residues: biodegradation and bioconversion by fungi.木质纤维素残渣：真菌的生物降解与生物转化
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Characterization of xylanase from Lentinus edodes M290 cultured on waste mushroom logs.香菇M290在废弃菇木上培养所产木聚糖酶的特性研究
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Cloning, characterization, and expression of xylanase A gene from Paenibacillus sp. DG-22 in Escherichia coli.芽孢杆菌属DG-22木聚糖酶A基因在大肠杆菌中的克隆、表征及表达
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Lentinus edodes and Pleurotus species lignocellulolytic enzymes activity in submerged and solid-state fermentation of lignocellulosic wastes of different composition.香菇和平菇在不同成分木质纤维素废弃物的深层发酵和固态发酵中木质纤维素酶的活性
Bioresour Technol. 2008 Feb;99(3):457-62. doi: 10.1016/j.biortech.2007.01.011. Epub 2007 Mar 12.
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Xylanases from fungi: properties and industrial applications.来自真菌的木聚糖酶：特性与工业应用
Appl Microbiol Biotechnol. 2005 Jun;67(5):577-91. doi: 10.1007/s00253-005-1904-7. Epub 2005 Jan 27.
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Thermal stability of beta-xylanases produced by different Thermomyces lanuginosus strains.不同羊毛嗜热丝孢菌菌株产生的β-木聚糖酶的热稳定性
Enzyme Microb Technol. 2000 Apr 1;26(7):502-508. doi: 10.1016/s0141-0229(99)00193-3.
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Characteristics and N-terminal amino acid sequence of a manganese peroxidase purified from Lentinula edodes cultures grown on a commercial wood substrate.从在商业木材基质上生长的香菇培养物中纯化得到的锰过氧化物酶的特性及N端氨基酸序列
Appl Microbiol Biotechnol. 1990 Jun;33(3):359-65. doi: 10.1007/BF00164536.
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.一种利用蛋白质 - 染料结合原理对微克级蛋白质进行定量的快速灵敏方法。
Anal Biochem. 1976 May 7;72:248-54. doi: 10.1016/0003-2697(76)90527-3.