School of Life Sciences, North Maharashtra University , P.B. No. 80, Jalgaon 425 001, MS , India.
Braz J Microbiol. 2012 Jan;43(1):348-55. doi: 10.1590/S1517-838220120001000041. Epub 2012 Jun 1.
Alkaliphilic cyanobacterial cultures were isolated from Lonar lake (MS, India). Among the set of cultures, Synechocystis sp, was studied for phycocyanin production. A maximum yield was obtained in BG-11 medium at optimized conditions (pH 10 and 16 h light). In order to increase the phycocyanin yield media optimization based on the eight media components a Plackett-Burman design of the 12 experimental trials was used. As per the analysis CaCl2, 2H2O and Na2CO3 have been found to be the most influencing media components at 95% significance. Further the optimum concentrations of these components were estimated following a Box Wilson Central Composite Design (CCD) with four star points and five replicates at the center points for each of two factors was adopted for optimization of these two media components. The results indicated that there was an interlinked influence of CaCl2, 2H2O and Na2CO3 on 98% significance. The maximum yield of phycocyanin (12% of dry wt) could be obtained at 0.058 g/l and 0.115 g/l of CaCl2, 2H2O and Na2CO3, respectively.
从印度马哈拉施特拉邦的洛纳尔湖分离到嗜堿性蓝藻培养物。在这组培养物中，研究了鱼腥藻属（Synechocystis sp.）的藻蓝蛋白生产。在优化条件（pH 10 和 16 小时光照）下，在 BG-11 培养基中获得最大产量。为了提高藻蓝蛋白的产量，基于 8 种培养基成分进行了基于 Plackett-Burman 设计的 12 次实验试验的培养基优化。根据分析，CaCl2、2H2O 和 Na2CO3 被发现是最具影响力的培养基成分，在 95%的置信水平下。进一步采用 Box-Wilson 中心复合设计（CCD），对这两种培养基成分进行优化，每个因素有四个星点和五个中心点重复，以估计这些成分的最佳浓度。结果表明，CaCl2、2H2O 和 Na2CO3 之间存在 98%显著的相互影响。在 0.058 g/L 和 0.115 g/L 的 CaCl2、2H2O 和 Na2CO3 下，藻蓝蛋白的最大产量（占干重的 12%）可以获得。
