National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi, Kerala, 682016, India.
Environ Sci Pollut Res Int. 2017 Dec;24(34):26763-26777. doi: 10.1007/s11356-017-0274-x. Epub 2017 Sep 29.
Diatoms hold great promise as potential sources of biofuel production. In the present study, the biomass and lipid production in the marine diatom Navicula phyllepta, isolated from Cochin estuary, India and identified as a potential biodiesel feedstock, were optimized using Plackett-Burman (PB) statistical experimental design followed by central composite design (CCD) and response surface methodology (RSM). The growth analyses of the isolate in different nitrogen sources, salinities and five different enriched sea water media showed the best growth in the cheapest medium with minimum components using urea as nitrogen source at salinity between 25 and 40 g kg. Plackett-Burman experimental analyses for screening urea, sodium metasilicate, sodium dihydrogen phosphate, ferric chloride, salinity, temperature, pH and agitation influencing lipid and biomass production showed that silicate and temperature had a positive coefficient on biomass production, and temperature had a significant positive coefficient, while urea and phosphate showed a negative coefficient on lipid content. A 2 factorial central composite design (FCCD) was used to optimize the concentration of the factors selected. The optimized media resulted in 1.62-fold increase (64%) in biomass (1.2 ± 0.08 g L) and 1.2-fold increase (22%) in estimated total lipid production (0.11 ± 0.003 g L) compared to original media within 12 days of culturing. A significantly higher biomass and lipid production in the optimized medium demands further development of a two-stage strategy of biomass production followed by induction of high lipid production under nutrient limitation or varying culture conditions for large-scale production of biodiesel from the marine diatom.
甲藻作为生物燃料生产的潜在来源具有巨大的潜力。在本研究中，通过 Plackett-Burman（PB）统计实验设计、中心复合设计（CCD）和响应面法（RSM），对从印度科钦河口分离出的海洋甲藻 Navicula phyllepta 的生物量和脂质生产进行了优化，该藻被鉴定为潜在的生物柴油原料。对分离株在不同氮源、盐度和五种不同富海水培养基中的生长分析表明，在最便宜的培养基中使用尿素作为氮源，盐度在 25 至 40 g kg 之间，使用的成分最少，生长最好。Plackett-Burman 实验分析筛选了影响脂质和生物量生产的尿素、偏硅酸钠、磷酸二氢钠、氯化铁、盐度、温度、pH 值和搅拌等因素，结果表明硅酸盐和温度对生物量生产有正系数，而温度对生物量生产有显著的正系数，而尿素和磷酸盐对脂质含量有负系数。采用 2 因子中心复合设计（FCCD）优化所选因素的浓度。优化后的培养基使生物量（1.2±0.08 g L）增加了 1.62 倍（64%），总估计脂质产量（0.11±0.003 g L）增加了 1.2 倍，与原始培养基相比，培养 12 天内的生物量和脂质产量分别增加了 1.62 倍（64%）和 1.2 倍（22%）。在优化后的培养基中，生物量和脂质产量显著提高，这需要进一步开发一种两步策略，即在营养限制或不同培养条件下进行生物量生产，然后诱导高脂质生产，以便从海洋甲藻大规模生产生物柴油。
