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从污水中分离的荧光假单胞菌噬菌体的特性和裂解活性。

Characterization and lytic activity of Pseudomonas fluorescens phages from sewage.

机构信息

Department of Biotechnology, PSG College of Technology , Coimbatore , India.

出版信息

Braz J Microbiol. 2012 Jan;43(1):356-62. doi: 10.1590/S1517-838220120001000042. Epub 2012 Jun 1.

Abstract

Pseudomonas fluorescens phages from sewage were tested against P. fluorescens isolates of soil and sewage. The phages were characterized as to host range, morphology, structural proteins and genome fingerprint. Of the seven phages isolated, one was found to be abundant in sewage (5.9×10(7) pfu/mL), having broad host range, and distinct protein and DNA profile when compared to the other six phages. DNA restriction and protein profiles of the phages and their morphology indicate the diversity in the sewage environment. None of the isolates from the rhizosphere regions of various cultivated soils were susceptible to phages isolated from sewage.

摘要

从污水中分离的荧光假单胞菌噬菌体被用于检测土壤和污水中的荧光假单胞菌分离株。噬菌体的宿主范围、形态、结构蛋白和基因组指纹图谱等特征被进行了研究。在所分离的七个噬菌体中,有一个在污水中含量丰富(5.9×10(7) pfu/mL),与其他六个噬菌体相比,具有广泛的宿主范围和独特的蛋白质和 DNA 图谱。噬菌体及其形态的 DNA 限制和蛋白质图谱表明,污水环境中存在多样性。从各种栽培土壤的根际区域分离到的分离株均不受从污水中分离到的噬菌体的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1323/3768991/f7bdbeab432c/bjm-43-356-g001.jpg

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