使用宫颈阴道样本直接浸渍 FTA 洗脱卡注入扩增反应可提高人乳头瘤病毒 DNA 的检测率。
Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA.
机构信息
Centro de Desenvolvimento Científico e Tecnológico, Fundação Estadual de Produção e Pesquisa em Saúde , Porto Alegre, RS , Brasil ; Universidade Luterana do Brasil , Canoas, RS , Brasil.
出版信息
Braz J Microbiol. 2012 Jan;43(1):389-92. doi: 10.1590/S1517-838220120001000047. Epub 2012 Jun 1.
Abstract
This study aimed to evaluate the use of the FTA elute card(TM) impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples.
摘要
本研究旨在评估直接使用 FTA elute 卡（TM）提取宫颈阴道样本进行 PCR 扩增检测 HPV-DNA 的应用。结果与参考技术进行了比较。该方法比制造商推荐的方案更有效，阳性样本的检出率从 54.2%提高到 91.7%。
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