滤纸在皮肤利什曼病诊断 PCR 中的性能。
Filter paper performance in PCR for cutaneous leishmaniasis diagnosis.
机构信息
Universidade Estadual de Maringá, Programa de Pós-Graduação em Biociências e Fisiopatologia, Maringá, PR, Brasil.
Universidade Estadual de Maringá, Departamento de Análises Clínicas e Biomedicina, Laboratório de Parasitologia Clínica, Maringá, PR, Brasil.
出版信息
Rev Soc Bras Med Trop. 2020 Dec 21;54:e00472020. doi: 10.1590/0037-8682-0047-2020. eCollection 2020.
INTRODUCTION
The objective of this study was to evaluate the performance of filter paper (FP) for lesion scraping collection in a polymerase chain reaction (PCR) for cutaneous leishmaniasis (CL) diagnosis.
METHODS
Lesion scrapings from 48 patients were collected and analyzed for PCR.
RESULTS
PCR with FP detected up to three Leishmania braziliensis promastigotes. Considering the direct search by microscopy or PCR of samples collected in STE buffer as standards, the sensitivity of PCR with FP was 100%.
CONCLUSIONS
FP can be useful for CL diagnosis in remote regions, allowing high sensitivity in the detection of the parasite by PCR.
简介
本研究旨在评估滤纸（FP）在聚合酶链反应（PCR）中用于皮肤利什曼病（CL）诊断时对病变刮取物收集的性能。
方法
对 48 例患者的病变刮取物进行采集并进行 PCR 分析。
结果
FP-PCR 可检测到多达三个巴西利什曼原虫前鞭毛体。考虑到以直接显微镜检查或 STE 缓冲液采集的样本作为标准进行 PCR，FP-PCR 的灵敏度为 100%。
结论
FP 可用于偏远地区的 CL 诊断，通过 PCR 检测寄生虫的灵敏度高。
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