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应用免疫磁珠分离-PCR 方法诊断犬钩端螺旋体病。

Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method.

机构信息

Laboratório de Imunologia Aplicada, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas , Pelotas, RS , Brasil ; Laboratório de Imunodiagnóstico, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas , Pelotas, RS , Brasil.

出版信息

Braz J Microbiol. 2012 Apr;43(2):602-5. doi: 10.1590/S1517-83822012000200023. Epub 2012 Jun 1.

DOI:10.1590/S1517-83822012000200023
PMID:24031870
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3768826/
Abstract

Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from dogs. It resulted in a significant improvement on sensitivity for diagnosis of canine leptospirosis.

摘要

PCR 诊断由于生物体液中存在物质而受到阻碍。在这里，我们报告了在 PCR 之前进行免疫磁珠分离步骤，这提高了从狗的血液和尿液样本中检测钩端螺旋体属的能力。这显著提高了犬钩端螺旋体病的诊断灵敏度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/3768826/5043bedebcf7/bjm-43-602-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/3768826/5043bedebcf7/bjm-43-602-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c60/3768826/5043bedebcf7/bjm-43-602-g001.jpg

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