Environmental and Advanced Parasitology Lab, Department of Zoology, Faculty of Science, Dayalbagh Educational Institute (Deemed University) , Dayalbagh, Agra - 282110 , India.
Braz J Microbiol. 2012 Apr;43(2):834-43. doi: 10.1590/S1517-83822012000200050. Epub 2012 Jun 1.
Majority of studies concerning the gene expression of Listeria monocytogenes have been done on pure culture states. Our objective was to study L.monocytogenes in a co-cultured state and to understand if microbes in their natural state of existence are different in their expression than that of the purely cultured lab grown forms. For a long period discussions have been on the expression of prfA, (which is a virulence gene regulator) in a mammalian host and its role in causing the switch from a saprophytic to pathogenic form of L.monocytogenes. We, in this paper for the first time report the expression of prfA and other virulence genes by L.monocytogenes under different extracellular conditions, and also as a pure culture biofilms, that is different from the previous reports. We also report that the expression of prfA seems to vary considerably when co-cultured with Bacillus subtilis.
大多数关于李斯特菌基因表达的研究都是在纯培养状态下进行的。我们的目的是研究共培养状态下的李斯特菌，并了解其在自然存在状态下的表达是否与纯培养实验室生长形式不同。长期以来，人们一直在讨论 prfA（一种毒力基因调节剂）在哺乳动物宿主中的表达及其在导致李斯特菌从腐生形式向致病性形式转变中的作用。在本文中，我们首次报道了李斯特菌在不同细胞外条件下的 prfA 和其他毒力基因的表达，以及作为纯培养生物膜的表达，这与之前的报道不同。我们还报告说，当与枯草芽孢杆菌共培养时，prfA 的表达似乎有很大的差异。
