利用流式细胞术检测与分析污水处理厂中两种血清型的氨氧化细菌。
Detection and analysis of two serotypes of ammonia-oxidizing bacteria in sewage plants by flow cytometry.
作者信息
Völsch A, Nader W F, Geiss H K, Nebe G, Birr C
机构信息
ORPEGEN, Medizinisch-Molekularbiologische Forschungsgesellschaft mbH, Heidelberg, Federal Republic of Germany.
出版信息
Appl Environ Microbiol. 1990 Aug;56(8):2430-5. doi: 10.1128/aem.56.8.2430-2435.1990.
Abstract
Two different serotypes of the genus Nitrosomonas were isolated from samples of the sewage plant Heidelberg. These nitrifiers were enumerated in activated sludge of various other sewage plants after immunofluorescent labeling and staining with propidium iodide by flow cytometry. The concentrations of these serotypes of Nitrosomonas spp. were in the range of 0.1 to 2%. Also, a test for the determination of the activity of ammonia-oxidizing bacteria was developed. Nitrite-oxidizing bacteria were specifically inhibited with sodium chlorate, and the activity of ammonia-oxidizing bacteria could be calculated from the increase of nitrite. Concentrations and activities of ammonia oxidizers were measured for a period of 6 months in the sewage plant Heidelberg. With one exception, activities and concentrations of ammonia-oxidizing bacteria decreased and increased in parallel.
摘要
从海德堡污水处理厂的样本中分离出了两种不同血清型的亚硝化单胞菌属。在用碘化丙啶进行免疫荧光标记并通过流式细胞术染色后，在其他各种污水处理厂的活性污泥中对这些硝化细菌进行了计数。这些亚硝化单胞菌属血清型的浓度在0.1%至2%的范围内。此外，还开发了一种测定氨氧化细菌活性的测试方法。用氯酸钠特异性抑制亚硝酸盐氧化细菌，氨氧化细菌的活性可根据亚硝酸盐的增加量来计算。在海德堡污水处理厂对氨氧化菌的浓度和活性进行了为期6个月的测量。除了一个例外，氨氧化细菌的活性和浓度呈平行下降和上升趋势。
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