Department of Biochemistry, University of Kerala , Thiruvananthapuram , India.
Pharm Biol. 2013 Dec;51(12):1552-8. doi: 10.3109/13880209.2013.802353. Epub 2013 Sep 13.
The anti-atherogenic effect of alkaloid fraction from Ruta graveolens Linn (Rutaceae) extract is suspected to be related to its activities of antioxidation and anti-inflammation.
This study investigated the efficacy of alkaloid fraction isolated from Ruta graveolens (AFR) in reducing oxidative damage and inflammation in hypercholesteremic rabbits.
The New Zealand white rabbits were randomly divided into three groups: Group I rabbits were fed with normal chow diet for 90 d. Group II rabbits were fed with 1% cholesterol-enriched diet. Group III rabbits were fed with 1% cholesterol-enriched diet together with AFR (10 mg/kg/daily for 90 d).
The results showed that on treatment with AFR significantly lowered the level of total cholesterol and LDL-C and showed an increment in the level of HDL-C. LD50 of the AFR in rats is greater than 525 mg/kg. Activities of antioxidant enzymes such as superoxide dismutase, catalase and glutathione peroxidase and GSH level were decreased in cholesterol-fed rabbit and supplementation of AFR significantly enhanced the activities of these antioxidant enzymes and GSH level. Increased activities of enzymes such as cyclooxygenase-2, 15-lipoxygenase and myeloperoxidase were significantly suppressed by AFR administration. The acute phase proteins, total WBC count and TBARS concentrations were significantly increased by hypercholesteromic diet, which were significantly decreased by AFR treatment. Histopathological studies of aorta in cholesterol-fed rabbit showed plaque formation and significant changes in aortic wall. Administration of AFR showed no changes in aortic wall.
AFR reduces oxidative stress and inflammation and reduces the aortic pathology in hypercholesteromic rabbits.
贯叶连翘生物碱部分的抗动脉粥样硬化作用可能与其抗氧化和抗炎作用有关。
本研究旨在探讨贯叶连翘生物碱部分（AFR）对高胆固醇血症兔氧化损伤和炎症的疗效。
新西兰白兔随机分为三组：第 1 组给予普通饲料 90d；第 2 组给予 1%胆固醇饲料；第 3 组给予 1%胆固醇饲料加 AFR（10mg/kg/d，90d）。
AFR 治疗组可显著降低总胆固醇和 LDL-C 水平，升高 HDL-C 水平。AFR 在大鼠中的 LD50 大于 525mg/kg。与胆固醇喂养兔相比，AFR 可显著增强超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶等抗氧化酶的活性和 GSH 水平。AFR 可显著抑制环氧化酶-2、15-脂氧合酶和髓过氧化物酶等酶的活性。高胆固醇血症饮食可显著增加急性期蛋白、总白细胞计数和 TBARS 浓度，而 AFR 治疗可显著降低这些指标。胆固醇喂养兔的主动脉组织病理学显示斑块形成和主动脉壁明显变化，而 AFR 治疗则无明显变化。
AFR 可降低氧化应激和炎症，减少高胆固醇血症兔的主动脉病理学改变。
