Ratheesh M, Shyni G L, Sindhu G, Helen A
Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram 695 581, India.
Exp Toxicol Pathol. 2011 Mar;63(3):285-90. doi: 10.1016/j.etp.2010.01.007. Epub 2010 Feb 18.
The purpose of the study was to investigate the efficacy of methanolic extract of Ruta graveolens L. in reducing oxidative damage, inflammation and aortic pathology in hypercholesteremic rats. For the study rats were divided into three groups - control group, hypercholesteremic group and treatment group (20 mg MER/kg/d orally) - and were fed for 90 days. Serum total cholesterol, LDL-C, total WBC count, CRP level, TBARS, atherogenic index, activities of COX, 15 LOX in monocyte and serum myeloperoxidase were increased in cholesterol fed rats. Activities of antioxidant enzymes and the concentration reduced glutathione in liver and heart tissue and serum HDL-C were decreased in cholesterol fed rats. The results showed that level of total cholesterol, LDL-C, atherogenic index was decreased and HDL-C was increased in MER treated rats. Activities of antioxidant enzymes were found to be increased and the activity of MPO, COX and 15 LOX were decreased on supplementation with MER. Concentration of TBARS and total WBC count were decreased and GSH was increased on supplementation with MER. Histopathology of aorta of cholesterol fed rat showed marked alterations whereas the aorta of MER administrated rat showed no significant changes. These results suggested that MER reduces oxidative stress, inflammation and aortic pathology in hypercholesteremic rats. Thus the plant may therefore be useful for therapeutic treatment of clinical conditions associated atherosclerosis.
本研究的目的是调查芸香甲醇提取物对降低高胆固醇血症大鼠氧化损伤、炎症和主动脉病变的疗效。在该研究中，大鼠被分为三组——对照组、高胆固醇血症组和治疗组（口服20毫克芸香甲醇提取物/千克/天），并喂养90天。喂食胆固醇的大鼠血清总胆固醇、低密度脂蛋白胆固醇、白细胞总数、CRP水平、丙二醛、动脉粥样硬化指数、单核细胞中COX、15-LOX的活性以及血清髓过氧化物酶均升高。喂食胆固醇的大鼠肝脏、心脏组织和血清高密度脂蛋白胆固醇中的抗氧化酶活性及还原型谷胱甘肽浓度降低。结果显示，芸香甲醇提取物治疗的大鼠总胆固醇、低密度脂蛋白胆固醇水平和动脉粥样硬化指数降低，高密度脂蛋白胆固醇升高。补充芸香甲醇提取物后，抗氧化酶活性增加，MPO、COX和15-LOX的活性降低。补充芸香甲醇提取物后，丙二醛浓度和白细胞总数降低，谷胱甘肽增加。喂食胆固醇的大鼠主动脉组织病理学显示有明显改变，而给予芸香甲醇提取物的大鼠主动脉无明显变化。这些结果表明，芸香甲醇提取物可减轻高胆固醇血症大鼠的氧化应激、炎症和主动脉病变。因此，该植物可能对治疗与动脉粥样硬化相关的临床病症有用。
