Ratheesh M, Shyni G L, Helen A
Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram 695581, India.
Inflammopharmacology. 2009 Apr;17(2):100-5. doi: 10.1007/s10787-009-8044-0.
Ruta graveolens L. (Rutaceae) are traditionally used for the treatment of rheumatism, arthritis and other inflammatory conditions in the traditional medicine of India. The purpose of this study was to investigate anti-inflammatory and anti-oxidant effects of methanolic extract of Ruta graveolens L. in adjuvant induced arthritis in rats. Methanolic extract of Ruta graveolens (MER) exhibited maximum percentage of oedema inhibition at a dose of 20 mg/kg on 21st day of adjuvant arthritis. The effect was higher than that of standard drug indomethacin. The activities of cycloxygenase-2 and myeloperoxidase and concentration of thiobarbituric acid reactive substance (TBARS) were decreased and the activities of antioxidant enzymes, vitamins C & E and reduced glutathione level were increased on treatment with MER. The increment in ESR and total WBC, reduction in RBC count and haemoglobin and aberrant changes to the C-reactive protein (CRP) and ceruloplasmin levels observed in the arthritic animals were also found to be significantly restored in MER treated rats. Histopathology of paw tissue showed decreased oedema formation and cellular infiltration on supplementation with MER. Thus the results demonstrated the potential beneficiary effect of methanolic extract of Ruta graveolens on adjuvant induced arthritis in rats.
芸香（芸香科）在印度传统医学中传统上用于治疗风湿病、关节炎和其他炎症性疾病。本研究的目的是调查芸香甲醇提取物对佐剂诱导的大鼠关节炎的抗炎和抗氧化作用。芸香甲醇提取物（MER）在佐剂性关节炎第21天以20mg/kg的剂量表现出最大的水肿抑制百分比。该效果高于标准药物吲哚美辛。用MER处理后，环氧化酶-2和髓过氧化物酶的活性以及硫代巴比妥酸反应性物质（TBARS）的浓度降低，抗氧化酶、维生素C和E的活性以及还原型谷胱甘肽水平升高。在患关节炎的动物中观察到的血沉速率（ESR）和白细胞总数增加、红细胞计数和血红蛋白降低以及C反应蛋白（CRP）和铜蓝蛋白水平的异常变化，在MER处理的大鼠中也被发现显著恢复。爪组织的组织病理学显示，补充MER后水肿形成和细胞浸润减少。因此，结果证明了芸香甲醇提取物对佐剂诱导的大鼠关节炎具有潜在的有益作用。
