Koener J F, Leong J A
Department of Microbiology, Oregon State University, Corvallis 97331-3804.
J Virol. 1990 Jan;64(1):428-30. doi: 10.1128/JVI.64.1.428-430.1990.
A cDNA fragment containing the gene encoding the glycoprotein of infectious hematopoietic necrosis virus was inserted into Autographa californica baculovirus vectors under the control of the polyhedrin promoter. A 66-kilodalton protein, identical in size to the glycosylated glycoprotein of infectious hematopoietic necrosis virus, was expressed at high levels in Spodoptera frugiperda cells infected with the recombinant viruses. The expressed protein reacted with antiserum to the glycoprotein on Western blots (immunoblots).
将含有传染性造血坏死病毒糖蛋白编码基因的cDNA片段，在多角体蛋白启动子的控制下插入苜蓿银纹夜蛾杆状病毒载体。一种大小为66千道尔顿的蛋白质，其大小与传染性造血坏死病毒的糖基化糖蛋白相同，在感染重组病毒的草地贪夜蛾细胞中高水平表达。在蛋白质印迹法（免疫印迹）中，表达的蛋白质与针对该糖蛋白的抗血清发生反应。
