Matsuura Y, Possee R D, Bishop D H
J Gen Virol. 1986 Aug;67 ( Pt 8):1515-29. doi: 10.1099/0022-1317-67-8-1515.
A DNA copy of the lymphocytic choriomeningitis virus (LCMV, WE strain)S RNA species has been inserted in both orientations into plasmids containing a 7.1 kb DNA sequence of the baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV). The inserts were placed behind the polyhedrin gene promoter of AcNPV and in the place of the nucleotide sequences including and flanking the polyhedrin gene translation initiation codon. The derived plasmids were used to obtain recombinant AcNPV viruses after transfection of Spodoptera frugiperda cells in the presence of infectious AcNPV DNA and the selection of polyhedrin-negative viruses. The expression of the two LCMV S-coded genes, the nucleoprotein and glycoprotein precursor, in S. frugiperda cells by the recombinant baculoviruses is described. Based on the results obtained with three different expression vectors, the site of insertion of the foreign genes in the 5' non-coding region of the polyhedrin gene appears to be an important determinant of the level of expression obtained.
淋巴细胞性脉络丛脑膜炎病毒（LCMV，WE株）S RNA种类的DNA拷贝已以两种方向插入到含有苜蓿银纹夜蛾核型多角体病毒（AcNPV）7.1 kb DNA序列的质粒中。插入片段置于AcNPV多角体蛋白基因启动子之后，并取代了包括多角体蛋白基因翻译起始密码子及其侧翼的核苷酸序列。在感染性AcNPV DNA存在的情况下，将得到的质粒转染草地贪夜蛾细胞，并筛选多角体蛋白阴性病毒，从而获得重组AcNPV病毒。本文描述了重组杆状病毒在草地贪夜蛾细胞中对LCMV S编码的两个基因（核蛋白和糖蛋白前体）的表达情况。基于用三种不同表达载体获得的结果，外源基因在多角体蛋白基因5'非编码区的插入位点似乎是所获表达水平的一个重要决定因素。
