通过聚合酶链反应鉴定口腔炎症病变中的放线菌 16S 核糖体 RNA 基因。
Identification of the actinomycete 16S ribosomal RNA gene by polymerase chain reaction in oral inflammatory lesions.
机构信息
Department of Oral Pathology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan; Department of Forensic Medicine, The Jikei University School of Medicine, Minato, Tokyo, Japan; Department of Diagnostic Pathology, Hospital of Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol. 2013 Oct;116(4):485-91. doi: 10.1016/j.oooo.2013.06.027.
OBJECTIVES
To examine the histopathological characteristics of inflammatory lesions containing Actinomyces based on DNA sequencing. Furthermore, case reports of actinomycosis in the maxillofacial region are summarized by a review of the literature.
STUDY DESIGN
The study comprised 12 cases of inflammatory lesions containing Actinomyces as diagnosed by DNA analysis. The average age of the subjects was 59 ± 15 years (6 males; 6 females).
RESULTS
The distribution of causative bacteria was: Actinomyces israelii in 9 cases, Actinomyces gerencseriae in 2 cases, and Actinomyces naeslundii in 1 case. Four cases diagnosed by DNA sequencing were positive for "Druse," a known morphological diagnostic characteristic of actinomycosis, and 8 cases lacked typical colony formation.
CONCLUSIONS
DNA analysis using paraffin-embedded samples is effective for both early and accurate diagnosis of oral lesions containing Actinomyces.
目的
通过 DNA 测序，研究含放线菌的炎性病变的组织病理学特征。此外，通过文献复习，总结了口腔颌面部放线菌病的病例报告。
研究设计
本研究共纳入 12 例经 DNA 分析诊断为含放线菌的炎性病变。患者平均年龄为 59±15 岁（6 名男性；6 名女性）。
结果
病原菌分布为：9 例为衣氏放线菌，2 例为杰氏放线菌，1 例为内氏放线菌。4 例经 DNA 测序诊断为“Druse”阳性，这是放线菌病的一种已知的形态学诊断特征，8 例则缺乏典型的菌落形成。
结论
使用石蜡包埋样本进行 DNA 分析，对口腔含放线菌病变的早期和准确诊断均有效。
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