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恶性疟原虫体外刺激人巨噬细胞产生肿瘤坏死因子

Tumor necrosis factor production by human macrophages stimulated in vitro by Plasmodium falciparum.

作者信息

Picot S, Peyron F, Vuillez J P, Barbe G, Marsh K, Ambroise-Thomas P

机构信息

Unité URA 1344 Centre National de la Recherche Scientifique, Hôpital A. Michallon, Grenoble, France.

出版信息

Infect Immun. 1990 Jan;58(1):214-6. doi: 10.1128/iai.58.1.214-216.1990.

Abstract

Production of tumor necrosis factor by human macrophages may be induced in vitro by cytoadherent and noncytoadherent strains of Plasmodium falciparum, with an optimal ratio of one to three parasitized erythrocytes per macrophage. Centrifuged and heated crude culture supernatants have the same effect, thus showing the existence of a thermostable soluble factor able to induce this expression. In vitro kinetic experiments have shown that the secretion of tumor necrosis factor appears early, with a maximal peak at 8 h.

摘要

恶性疟原虫的细胞黏附性和非细胞黏附性菌株在体外可诱导人巨噬细胞产生肿瘤坏死因子,最佳比例为每个巨噬细胞有1至3个被寄生的红细胞。经离心和加热的粗培养上清液有相同作用,从而表明存在一种能诱导这种表达的热稳定可溶性因子。体外动力学实验表明,肿瘤坏死因子的分泌出现较早,在8小时时达到最大峰值。

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