Université de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France; VEOLIA Environnement, Centre de Recherche sur l'Eau, Chemin de la Digue, BP 76, F-78603 Maisons-Laffitte Cedex, France.
Bioresour Technol. 2013 Nov;148:30-8. doi: 10.1016/j.biortech.2013.08.120. Epub 2013 Aug 26.
In this study a complementary modeling and experimental approach was used to explore how growth controls the NADPH generation and availability, and the resulting impact on PHB (polyhydroxybutyrate) yields and kinetics. The results show that the anabolic demand allowed the NADPH production through the Entner-Doudoroff (ED) pathway, leading to a high maximal theoretical PHB production yield of 0.89 C mole C mole(-1); whereas without biomass production, NADPH regeneration is only possible via the isocitrate dehydrogenase leading to a theoretical yield of 0.67 C mole C mole(-1). Furthermore, the maximum specific rate of NADPH produced at maximal growth rate (to fulfil biomass requirement) was found to be the maximum set in every conditions, which by consequence determines the maximal PHB production rate. These results imply that sustaining a controlled residual growth improves the PHB specific production rate without altering production yield.
在这项研究中，采用了一种补充的建模和实验方法来探索生长如何控制 NADPH 的产生和可用性，以及由此对 PHB（聚羟基丁酸酯）产率和动力学的影响。结果表明，合成代谢需求允许通过 Entner-Doudoroff（ED）途径产生 NADPH，从而导致高达 0.89 C 摩尔 C 摩尔（-1）的最大理论 PHB 产率；而没有生物量的产生，NADPH 的再生只能通过异柠檬酸脱氢酶进行，导致理论产率为 0.67 C 摩尔 C 摩尔（-1）。此外，在最大生长速率下（满足生物量需求）产生的 NADPH 的最大比速率被发现是在每种条件下的最大设定值，这反过来又决定了最大 PHB 生产速率。这些结果表明，维持受控的剩余生长可以提高 PHB 的比生产速率，而不改变产率。
