Gugulothu Dalapathi, Desai Preshita, Patravale Vandana
Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai 400019, India.
Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai 400019, India
J Chromatogr Sci. 2014 Sep;52(8):872-9. doi: 10.1093/chromsci/bmt131. Epub 2013 Sep 12.
A simple, rapid, sensitive and specific liquid chromatographic method was developed and validated for the determination of curcumin in human plasma. Berberine was used as the internal standard. Chromatographic separation was achieved on a Zorbax Eclipse C18 column at 40 °C, with a mobile phase consisting of 1% acetic acid (pH 3 adjusted with 50% triethanolamine): acetonitrile (55:45), at a flow rate of 1.25 mL/min. The method was validated for precision, accuracy, linearity, lower limit of quantification (LLOQ) and extraction efficiency according to the International Conference on Harmonization guidelines. The method was successfully developed with an LLOQ of 10 ng/mL and a runtime of 9 min. Linearity range was from 10 to 1000 ng/mL. Curcumin and Berberine were well separated with retention times of 8.2 ± 0.2 and 1.4 ± 0.1 min, respectively. Further, the method was successfully employed to study the pharmacokinetic parameters of curcumin, following oral administration of curcumin-loaded hydroxy propyl cellulose (HPC) nanoparticles and curcumin suspension in female Wistar rats. Curcumin-loaded HPC nanoparticles (Cmax: 106.01 ± 20.11 ng/mL) showed significant improvement in pharmacokinetic parameters when compared with curcumin suspension (Cmax: 30.13 ± 0.47 ng/mL) indicating 43.73-fold increase in relative bioavailability.
建立并验证了一种简单、快速、灵敏且特异的液相色谱法，用于测定人血浆中的姜黄素。以小檗碱为内标。在40℃下于Zorbax Eclipse C18柱上实现色谱分离，流动相由1%乙酸（用50%三乙醇胺调至pH 3）：乙腈（55:45）组成，流速为1.25 mL/min。根据国际协调会议指南，对该方法的精密度、准确度、线性、定量下限（LLOQ）和提取效率进行了验证。该方法成功开发，LLOQ为10 ng/mL，运行时间为9分钟。线性范围为10至1000 ng/mL。姜黄素和小檗碱分离良好，保留时间分别为8.2±0.2分钟和1.4±0.1分钟。此外，该方法成功用于研究雌性Wistar大鼠口服载姜黄素羟丙基纤维素（HPC）纳米粒和姜黄素混悬液后姜黄素的药代动力学参数。与姜黄素混悬液（Cmax：30.13±0.47 ng/mL）相比，载姜黄素HPC纳米粒（Cmax：106.01±20.11 ng/mL）的药代动力学参数有显著改善，表明相对生物利用度提高了43.73倍。
