Kłodińska Ewa, Kupczyk Wojciech, Jackowski Marek, Buszewski Boguslaw
Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Toruń, Poland.
Electrophoresis. 2013 Dec;34(22-23):3206-13. doi: 10.1002/elps.201300263. Epub 2013 Sep 11.
A surgical site infection (SSI) is an infection that occurs after surgery in the part of the body where the surgery took place. A SSI may range from a spontaneously limited wound discharge within 7-10 days of an operation to a life-threatening postoperative complication, such as a sternal infection after open heart surgery. Most surgical site infections are caused by contamination of an incision with microorganisms from the patient's own body during surgery. From the analytical point of view the complex natures of these samples as well as the low concentrations of analytes require a system with high sensitivity and efficiency. Such situation require a technique such as capillary electrophoresis which is a powerful and versatile separation technique that promises to rival high performance liquid chromatography when applied to the separation of both charged and neutral species. During the study, it has been demonstrated that CZE identifying characteristics of such groups of pathogens such as bacteria G (+) and different species of bacteria G (-) and also develop weekly individual profiles for patients after application of antibiotics. This was done in order to show the impact of antibiotic therapy in change "numbers" of bacteria present in the wound after surgery. The method proved to be the ideal straight specificity in the case of E. coli (100%). Finally analysis of the spectra and the second derivatives of the UV-Vis spectra confirmed the similarity in the profiles and showed that the CZE is a great method for fast screening test in bacterial infection.
手术部位感染（SSI）是指在手术部位进行手术后发生的感染。SSI的范围可以从手术后7至10天内自发局限的伤口渗液到危及生命的术后并发症，如心脏直视手术后的胸骨感染。大多数手术部位感染是由手术过程中患者自身身体的微生物污染切口所致。从分析的角度来看，这些样本的复杂性质以及分析物的低浓度需要一个具有高灵敏度和高效率的系统。这种情况需要一种技术，如毛细管电泳，它是一种强大且通用的分离技术，在用于分离带电和中性物质时有望与高效液相色谱相媲美。在研究过程中，已证明毛细管区带电泳（CZE）能够识别诸如革兰氏阳性菌和不同种类革兰氏阴性菌等病原体组的特征，并且在应用抗生素后还能为患者建立每周的个体图谱。这样做是为了显示抗生素治疗对手术后伤口中存在的细菌“数量”变化的影响。该方法在大肠杆菌检测中被证明具有理想的特异性（100%）。最后，对紫外可见光谱及其二阶导数的分析证实了图谱的相似性，并表明CZE是细菌感染快速筛查检测的一种很好的方法。
