Department of General, Gastroenterological and Oncological Surgery, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, Torun, Poland.
Med Sci Monit. 2011 Oct;17(10):MT91-6. doi: 10.12659/msm.881967.
Escherichia coli is a Gram-negative bacterium which is a basic, symbiotic element of the physiological flora of the large intestine of humans and warm-blooded animals. However, in specific cases it may become a very dangerous pathogen (eg, diarrhoea, infection of the urinary tract, lungs, and generalized infections). Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.
MATERIAL/METHODS: We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour. Altogether, 30 E. coli-infected wounds and ulcerations were examined, comparing the results obtained by classical culture method with the result of capillary zone electrophoresis (CZE) electropherogram.
The method, which does not require any preparation of the sample, achieved 86.7% sensitivity and 85%specificity in the examined clinical material (infections of surgical wounds).
The obtained results enable reliable, very fast testing for E. coli as a pathogen.
大肠杆菌是一种革兰氏阴性细菌，是人类和温血动物大肠生理菌群的基本共生元素。然而，在特定情况下，它可能成为一种非常危险的病原体（例如腹泻、尿路感染、肺部感染和全身性感染）。早期发现作为传染病的病原体有助于实现最佳的治疗效果；然而，经典的微生物学测试从取样到诊断至少需要 24 小时。
材料/方法：我们提出了一种独特的基于 CZE 技术的解决方案，能够在半小时内确定研究样本中是否存在大肠杆菌。总共检查了 30 例大肠杆菌感染的伤口和溃疡，将经典培养方法获得的结果与毛细管区带电泳（CZE）电泳图谱的结果进行了比较。
该方法不需要对样品进行任何预处理，在检查的临床材料（外科伤口感染）中达到了 86.7%的灵敏度和 85%的特异性。
获得的结果能够可靠、快速地检测出大肠杆菌作为病原体。
