Losurdo Pasquale, Grillo Andrea, Panizon Emiliano, Gortan Cappellari Gianluca, Fabris Bruno, Bardelli Moreno, Biolo Gianni, Zanetti Michela, Carretta Renzo
1Department of Medicine, Surgery and Health Sciences, U.C.O. Medicina Clinica, University of Trieste, Trieste, Italy.
J Cardiovasc Pharmacol Ther. 2014 Jan;19(1):114-20. doi: 10.1177/1074248413500716. Epub 2013 Sep 13.
Menopause is associated with increased arterial stiffness, an independent marker of cardiovascular risk. Omega-3 polyunsaturated fatty acids (N3-PUFAs) are thought to have multiple cardiovascular benefits, including prevention of arterial stiffness. We investigated whether treatment with N3-PUFA prevents increase in arterial stiffness in ovariectomized rats, an animal model of experimental menopause.
A total of 43 Wistar rats, 2 months old, were divided into 3 groups, control, sham surgery, normal diet (CTRL, n = 15); ovariectomy, normal diet (OVX, n = 14); and ovariectomy with N3-PUFA supplementation (0.8 g/kg/d in daily gavages administration; OVX + O3, n = 14). Two months after surgery, carotid-femoral pulse wave velocity (PWV) and arterial blood pressure (BP) were measured by carotid and femoral cannulation. Aortic morphometric measurements were performed after dissection.
Ovariectomy caused a significant increase in BP (P < .05), PWV (P < .0001), and elastic modulus (P = .001) compared to CTRL. After ovariectomy, N3-PUFA supplementation completely prevented increase in arterial stiffness (P < .0001 vs OVX) and BP (P < .05 vs OVX) and resulted in a significant increase in body weight and aortic thickness.
In an experimental model of menopause, N3-PUFA supplementation prevents arterial stiffening and other vascular changes induced by ovariectomy. These results represent a therapeutic benefit of N3-PUFAs in prevention of postmenopausal cardiovascular disease.
绝经与动脉僵硬度增加相关，动脉僵硬度是心血管风险的独立标志物。ω-3多不饱和脂肪酸（N3-PUFA）被认为具有多种心血管益处，包括预防动脉僵硬度增加。我们研究了N3-PUFA治疗是否能预防去卵巢大鼠（一种实验性绝经的动物模型）的动脉僵硬度增加。
将43只2月龄的Wistar大鼠分为3组，即对照组、假手术组、正常饮食（CTRL，n = 15）；去卵巢组、正常饮食（OVX，n = 14）；以及去卵巢并补充N3-PUFA组（每日灌胃给药0.8 g/kg/d；OVX + O3，n = 14）。术后两个月，通过颈动脉和股动脉插管测量颈股脉搏波速度（PWV）和动脉血压（BP）。解剖后进行主动脉形态学测量。
与CTRL组相比，去卵巢导致BP（P <.05）、PWV（P <.0001）和弹性模量（P =.001）显著增加。去卵巢后，补充N3-PUFA完全预防了动脉僵硬度（与OVX组相比，P <.0001）和BP（与OVX组相比，P <.05）的增加，并导致体重和主动脉厚度显著增加。
在绝经实验模型中，补充N3-PUFA可预防去卵巢引起的动脉僵硬和其他血管变化。这些结果表明N3-PUFA在预防绝经后心血管疾病方面具有治疗益处。
