State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Advanced Materials Laboratory, Fudan University, 200433, Shanghai, China.
Adv Mater. 2013 Oct 4;25(37):5257-86. doi: 10.1002/adma.201301762. Epub 2013 Aug 16.
Cell-material interactions constitute a key fundamental topic in biomaterials study. Various cell cues and matrix cues as well as soluble factors regulate cell behaviors on materials. These factors are coupled with each other as usual, and thus it is very difficult to unambiguously elucidate the role of each regulator. The recently developed material techniques of surface patterning afford unique ways to reveal the underlying science. This paper reviews the pertinent material techniques to fabricate patterns of microscale and nanoscale resolutions, and corresponding cell studies. Some issues are emphasized, such as cell localization on patterned surfaces of chemical contrast, and effects of cell shape, cell size, cell-cell contact, and seeding density on differentiation of stem cells. Material cues to regulate cell adhesion, cell differentiation and other cell events are further summed up. Effects of some physical properties, such as surface topography and matrix stiffness, on cell behaviors are also discussed; nanoscaled features of substrate surfaces to regulate cell fate are summarized as well. The pertinent work sheds new insight into the cell-material interactions, and is stimulating for biomaterial design in regenerative medicine, tissue engineering, and high-throughput detection, diagnosis, and drug screening.
细胞-材料相互作用是生物材料研究中的一个关键基础课题。各种细胞线索和基质线索以及可溶性因子调节细胞在材料上的行为。这些因素通常相互耦合，因此很难明确阐明每个调节剂的作用。最近开发的表面图案化材料技术提供了揭示潜在科学的独特方法。本文综述了用于制造微尺度和纳米尺度分辨率图案的相关材料技术及其相应的细胞研究。强调了一些问题，例如化学对比图案化表面上的细胞定位，以及细胞形状、细胞大小、细胞间接触和接种密度对干细胞分化的影响。进一步总结了调节细胞黏附、细胞分化和其他细胞事件的材料线索。还讨论了一些物理性质（如表面形貌和基质硬度）对细胞行为的影响；总结了基底表面的纳米级特征来调节细胞命运。相关工作为细胞-材料相互作用提供了新的见解，并为再生医学、组织工程以及高通量检测、诊断和药物筛选中的生物材料设计提供了新的思路。
