Vostatek Matthias, Verin Elettra, Tamm Marvin, Rothbauer Mario, Toegel Stefan, Moscato Francesco
Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Waehringer Guertel 18-20/4L, Vienna, 1090, Austria.
Austrian Cluster for Tissue Regeneration, Donaueschingenstrasse 13, Vienna, 1200, Austria.
Macromol Biosci. 2025 Feb;25(2):e2400311. doi: 10.1002/mabi.202400311. Epub 2024 Sep 5.
The attributes of implant surfaces are pivotal for successful osseointegration. Among surface engineering strategies, microtopography stands out as a promising approach to promote early cellular interactions. This study aims to design and craft a novel biomimetic osteon-like surface modification and to compare its impact on human mesenchymal stem cells (hMSCs) with four established topographies: blank, inverted pyramids, protrusions, and grooves. Poly-ε-caprolactone samples are fabricated using 2-photon-polymerization and soft lithography, prior to analysis via scanning electron microscopy (SEM), water contact angle (WCA), and protein adsorption assays. Additionally, cellular responses including cell attachment, proliferation, morphology, cytoskeletal organization, and osteogenic differentiation potential are evaluated. SEM confirms the successful fabrication of microtopographies, with minimal effect on WCA and protein adsorption. Cell attachment experiments demonstrate a significant increase on the osteon-like structure, being three times higher than on the blank. Proliferation assays indicate a fourfold increase with osteon-like microtopography compared to the blank, while ALP activity is notably elevated with osteon-like microtopography at days 7 (threefold increase over blank) and 14 (fivefold increase over blank). In conclusion, the novel biomimetic osteon-like structure demonstrates favorable responses from hMSCs, suggesting potential for promoting successful implant integration in vivo.
种植体表面特性对于成功的骨结合至关重要。在表面工程策略中，微观形貌作为促进早期细胞相互作用的一种有前景的方法脱颖而出。本研究旨在设计并制作一种新型的仿生骨单位样表面改性，并将其对人间充质干细胞（hMSCs）的影响与四种已确立的形貌进行比较：空白、倒金字塔、突起和沟槽。在通过扫描电子显微镜（SEM）、水接触角（WCA）和蛋白质吸附测定进行分析之前，使用双光子聚合和软光刻技术制备聚ε-己内酯样品。此外，还评估了包括细胞附着、增殖、形态、细胞骨架组织和成骨分化潜能在内的细胞反应。SEM证实了微观形貌的成功制备，对WCA和蛋白质吸附的影响最小。细胞附着实验表明，在骨单位样结构上细胞附着显著增加，比空白组高出三倍。增殖测定表明，与空白组相比，骨单位样微观形貌使增殖增加了四倍，而在第7天（比空白组增加三倍）和第14天（比空白组增加五倍）时，骨单位样微观形貌使碱性磷酸酶活性显著升高。总之，新型仿生骨单位样结构显示出hMSCs的良好反应，表明其在体内促进种植体成功整合方面具有潜力。
