Chemistry Department, University of Central Florida, 4000 Central Florida Blvd, Orlando, FL 32816 (USA).
Angew Chem Int Ed Engl. 2013 Sep 27;52(40):10586-8. doi: 10.1002/anie.201303919. Epub 2013 Sep 3.
Sixty-four DNA strands hybridize to 16S rRNA to form 32 deoxyribozyme catalytic cores that produce a fluorescent signal. The approach allows detection of 0.6 pM 16S rRNA, or about 3×10(4) bacterial cells in a PCR-free format.
64 条 DNA 链与 16S rRNA 杂交形成 32 个脱氧核酶催化核心,产生荧光信号。该方法可在无 PCR 格式下检测到 0.6 pM 的 16S rRNA,或约 3×10(4)个细菌细胞。