FaptaSyme：一种将单体/寡聚物非选择性适配体传感器转变为寡聚物选择性传感器的策略。
FaptaSyme: A Strategy for Converting a Monomer/Oligomer-Nonselective Aptameric Sensor into an Oligomer-Selective One.
作者信息
Evangelista Baggio A, Kim Yoon-Seong, Kolpashchikov Dmitry M
机构信息
Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, 32816, USA.
Chemistry Department, University of Central Florida, Orlando, FL, 32816 Florida, USA.
出版信息
Chembiochem. 2018 Apr 26. doi: 10.1002/cbic.201800017.
Abstract
Aptameric sensors can bind molecular targets and produce output signals, a phenomenon that is used in bioassays. In some cases, it is important to distinguish between monomeric and oligomeric forms of a target. Here, we propose a strategy to convert a monomer/oligomer-nonselective sensor into an oligomer-selective sensor. We designed an aptazyme that produced a high fluorescent output in the presence of oligomeric α-synuclein (a molecular marker of Parkinson's disease) but not its monomeric form. The strategy is potentially useful in the design of point-of-care tests for the diagnosis of neurodegenerative diseases.
摘要
适体传感器可以结合分子靶标并产生输出信号，这一现象被用于生物测定。在某些情况下，区分靶标的单体形式和寡聚体形式很重要。在此，我们提出了一种将单体/寡聚体非选择性传感器转变为寡聚体选择性传感器的策略。我们设计了一种核酸适体酶，它在存在帕金森病的分子标志物——寡聚体α-突触核蛋白时会产生高荧光输出，但在其单体形式存在时则不会。该策略在设计用于诊断神经退行性疾病的即时检测方面可能具有实用性。
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