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构巢曲霉npeA基因座由青霉素生物合成所需的三个相邻基因组成。

The Aspergillus nidulans npeA locus consists of three contiguous genes required for penicillin biosynthesis.

作者信息

MacCabe A P, Riach M B, Unkles S E, Kinghorn J R

机构信息

Molecular Genetics Unit, University of St. Andrews, Fife, UK.

出版信息

EMBO J. 1990 Jan;9(1):279-87. doi: 10.1002/j.1460-2075.1990.tb08106.x.

DOI:10.1002/j.1460-2075.1990.tb08106.x
PMID:2403928
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC551659/
Abstract

Clones of Aspergillus nidulans genomic DNA spanning 20 kb have been isolated and shown by a combination of classical and molecular genetic means to represent the npeA locus, previously found to be one of four loci (npeA, npeB, npeC and npeD) involved in the synthesis of penicillin. As well as containing the gene encoding the second enzyme for penicillin biosynthesis, namely isopenicillin N synthetase (IPNS) (designated ipnA), our results show that these clones (pSTA200, pSTA201 and pSTA207) contain two more genes to form a cluster of three contiguous penicillin biosynthetic genes. Our evidence suggests that these genes encode delta (L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACVS) and acyl transferase (ACYT) (designated acvA and acyA respectively), the first and third enzymes required for penicillin biosynthesis, with the gene order being acvA-ipnA-acyA. Transcripts have been identified for the three genes and their approximate sizes determined--acvA 9.5 kb, ipnA 1.4 kb and acyA 1.6 kb. All three mRNA species are observed in cells grown in fermentation medium but not in cells grown in minimal medium, suggesting that the control of penicillin biosynthesis is, in part, at the level of mRNA accumulation. Finally our results show that acvA and ipnA genes are divergently transcribed, whilst acyA is transcribed in the same orientation as ipnA.

摘要

已分离出构巢曲霉基因组DNA跨度为20 kb的克隆，并通过经典遗传学和分子遗传学方法相结合的方式表明其代表npeA基因座，该基因座先前被发现是参与青霉素合成的四个基因座（npeA、npeB、npeC和npeD）之一。除了包含编码青霉素生物合成第二种酶即异青霉素N合成酶（IPNS）（命名为ipnA）的基因外，我们的结果表明这些克隆（pSTA200、pSTA201和pSTA207）还包含另外两个基因，形成了一个由三个相邻青霉素生物合成基因组成的簇。我们的证据表明，这些基因编码δ（L-α-氨基己二酰基）-L-半胱氨酰-D-缬氨酸合成酶（ACVS）和酰基转移酶（ACYT）（分别命名为acvA和acyA），它们是青霉素生物合成所需的第一种和第三种酶，基因顺序为acvA-ipnA-acyA。已鉴定出这三个基因的转录本，并确定了其大致大小——acvA为9.5 kb，ipnA为1.4 kb，acyA为1.6 kb。在发酵培养基中生长的细胞中观察到了所有三种mRNA种类，但在基本培养基中生长的细胞中未观察到，这表明青霉素生物合成的调控部分是在mRNA积累水平上。最后，我们的结果表明acvA和ipnA基因是反向转录的，而acyA与ipnA转录方向相同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccd0/551659/49200ad93344/emboj00228-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccd0/551659/35bdd6b8d7e2/emboj00228-0274-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccd0/551659/652be9ddfee6/emboj00228-0275-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccd0/551659/52dc25a30edb/emboj00228-0276-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccd0/551659/3dc62fec49ae/emboj00228-0277-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccd0/551659/d238dd2066ef/emboj00228-0278-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccd0/551659/49200ad93344/emboj00228-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccd0/551659/35bdd6b8d7e2/emboj00228-0274-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccd0/551659/652be9ddfee6/emboj00228-0275-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccd0/551659/52dc25a30edb/emboj00228-0276-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccd0/551659/3dc62fec49ae/emboj00228-0277-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccd0/551659/d238dd2066ef/emboj00228-0278-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccd0/551659/49200ad93344/emboj00228-0279-a.jpg

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