Bernhard Nocht Institute for Tropical Medicine Hamburg; Molecular Entomology Group, Hamburg, Germany.
PLoS One. 2013 Sep 11;8(9):e71832. doi: 10.1371/journal.pone.0071832. eCollection 2013.
Mosquitoes and other arthropods may transmit medically important pathogens, in particular viruses such as West Nile virus. The presence of suitable hosts and competent vectors for those zoonotic viruses is essential for an enzootic transmission, which is a prerequisite for epidemics. To establish reliable risk projections, it is an urgent need for an exact identification of mosquito species, which is especially challenging in the case of sibling species, such as Culex. pipiens pipiens biotypes pipiens and molestus. To facilitate detection of different Culex pipiens forms and their hybrids we established a multiplex real-time PCR. Culex pipiens samples were obtained by egg raft collection and rearing until imago stage or adult sampling using CO2 baited traps and gravid traps. In total, we tested more than 16,500 samples collected all over Germany in the years 2011 and 2012. The predominant species in Germany are Culex pipiens pipiens biotype pipiens and Culex. torrentium, but we also detected Culex pipiens pipiens biotype molestus and hybrids of the two pipiens biotypes at sites where both species occur sympatrically. This report of a potentially important bridge vector for West Nile virus might have major impact in the risk projections for West Nile virus in Germany.
蚊子和其他节肢动物可能会传播具有医学重要性的病原体，特别是西尼罗河病毒等病毒。对于那些人畜共患的病毒来说，合适的宿主和媒介是地方性传播的必要条件，而地方性传播是流行病的前提。为了建立可靠的风险预测，准确识别蚊子种类是当务之急，而在像库蚊这样的姐妹种情况下，这尤其具有挑战性。为了方便检测不同的库蚊形态及其杂种，我们建立了多重实时 PCR。通过卵筏收集和饲养，直到若虫阶段或使用 CO2 诱捕器和诱捕器进行成虫采样，获得库蚊样本。总的来说，我们测试了 2011 年和 2012 年在德国各地收集的 16500 多个样本。德国的主要物种是库蚊 pipiens pipiens 生物型 pipiens 和库蚊 torrentium，但我们也在两种库蚊生物型共存的地点检测到了库蚊 pipiens pipiens 生物型 molestus 和杂种。本报告中提到的西尼罗河病毒的潜在重要桥梁媒介可能会对德国的西尼罗河病毒风险预测产生重大影响。
