Jiangsu Key Laboratory of New Power Batteries, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, P.R. China.
Analyst. 2013 Nov 7;138(21):6501-10. doi: 10.1039/c3an01375h.
Lung cancer is the leading cause of cancer death worldwide. Its early detection is of paramount importance for diagnosis, classification, treatment, and improvement of survivorship. However, current methods are not sensitive enough to detect lung cancer in its nascent stage. We reported an aptamer-Ag-Au shell-core nanostructure-based surface-enhanced Raman scattering (SERS) assay for sensitive and specific detection, and near-infrared (NIR) photothermal therapy of lung adenocarcinoma cells (A549 cells). The nanostructures target the cells with high affinity and specificity via the specific interaction between the aptamer (a 45-base oligonucleotide) and the cell, and distinguish A549 cells from other types of cancer cells (HeLa and MCF-7 cells) and subtypes of lung cancer cells (NCI-H157, NCI-H520, NCI-H1299, and NCI-H446 cells). The nanostructures have a high capability to absorb NIR irradiation and are able to perform photothermal therapy of the cells at a very low irradiation power density (0.20 W cm(-2)) without destroying the healthy cells and the surrounding normal tissues. In addition, the nanostructures exhibit a high SERS activity. Based on the SERS signal of the labeled Raman reporter (Rh6G molecules), we can specifically detect A549 cells at a very low abundance (~10 cells per mL) and monitor the therapy process of the cancer cells. Therefore, this nanostructure-based SERS assay has great potential in specific recognition, sensitive detection, and effective photothermal therapy of lung cancer.
肺癌是全球癌症死亡的主要原因。早期发现对诊断、分类、治疗和提高生存率至关重要。然而，目前的方法还不够敏感，无法在早期阶段检测到肺癌。我们报道了一种基于适配体-Ag-Au 核壳纳米结构的表面增强拉曼散射（SERS）分析方法，用于敏感和特异性检测肺腺癌细胞（A549 细胞），并进行近红外（NIR）光热治疗。这些纳米结构通过适配体（一种 45 个碱基的寡核苷酸）与细胞的特异性相互作用，以高亲和力和特异性靶向细胞，并将 A549 细胞与其他类型的癌细胞（HeLa 和 MCF-7 细胞）和不同亚型的肺癌细胞（NCI-H157、NCI-H520、NCI-H1299 和 NCI-H446 细胞）区分开来。这些纳米结构具有很强的吸收近红外辐射的能力，能够在非常低的辐照功率密度（0.20 W cm(-2)）下对细胞进行光热治疗，而不会破坏健康细胞和周围正常组织。此外，这些纳米结构还表现出很高的 SERS 活性。基于标记的拉曼报告分子（Rh6G 分子）的 SERS 信号，我们可以在非常低的丰度（~10 个细胞/mL）下特异性地检测 A549 细胞，并监测癌细胞的治疗过程。因此，这种基于纳米结构的 SERS 分析方法在肺癌的特异性识别、敏感检测和有效光热治疗方面具有很大的潜力。
