Department of Biotechnology and Cell Biology, Medical College, University of Information Technology and Management, St. Sucharskiego 2, 35-225, Rzeszów, Poland.
Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, St. Pigonia 1, 35-310, Rzeszów, Poland.
Sci Rep. 2021 Jun 29;11(1):13451. doi: 10.1038/s41598-021-92812-w.
Drug-resistance of bacteria is an ongoing problem in hospital treatment. The main mechanism of bacterial virulency in human infections is based on their adhesion ability and biofilm formation. Many approaches have been invented to overcome this problem, i.e. treatment with antibacterial biomolecules, which have some limitations e.g. enzymatic degradation and short shelf stability. Silver nanoparticles (AgNPs) may be alternative to these strategies due to their unique and high antibacterial properties. Herein, we report on yeast Saccharomyces cerevisiae extracellular-based synthesis of AgNPs. Transmission electron microscopy (TEM) revealed the morphology and structure of the metallic nanoparticles, which showed a uniform distribution and good colloid stability, measured by hydrodynamic light scattering (DLS). The energy dispersive X-ray spectroscopy (EDS) of NPs confirms the presence of silver and showed that sulfur-rich compounds act as a capping agent being adsorbed on the surface of AgNPs. Antimicrobial tests showed that AgNPs inhibit the bacteria growth, while have no impact on fungi growth. Moreover, tested NPs was characterized by high inhibitory potential of bacteria biofilm formation but also eradication of established biofilms. The cytotoxic effect of the NPs on four mammalian normal and cancer cell lines was tested through the metabolic activity, cell viability and wound-healing assays. Last, but not least, ability to deep penetration of the silver colloid to the root canal was imaged by scanning electron microscopy (SEM) to show its potential as the material for root-end filling.
细菌耐药性是医院治疗中一个持续存在的问题。细菌在人体感染中的毒力主要基于其黏附能力和生物膜形成。为了解决这个问题，人们发明了许多方法，例如使用具有抗菌作用的生物分子进行治疗，但这些方法存在一些局限性，如酶降解和货架期稳定性短。由于具有独特的高抗菌特性，银纳米粒子（AgNPs）可能是这些策略的替代方法。在本研究中，我们报告了酵母酿酒酵母细胞外合成 AgNPs。透射电子显微镜（TEM）揭示了金属纳米粒子的形态和结构，其通过动态光散射（DLS）显示出均匀的分布和良好的胶体稳定性。纳米粒子的能谱（EDS）证实了银的存在，并表明富含硫的化合物作为一种包覆剂吸附在 AgNPs 表面。抗菌试验表明，AgNPs 抑制细菌生长，但对真菌生长没有影响。此外，所测试的纳米粒子具有抑制细菌生物膜形成的高潜力，并且能够消除已建立的生物膜。通过代谢活性、细胞活力和划痕愈合试验，测试了纳米粒子对四种哺乳动物正常和癌细胞系的细胞毒性作用。最后但同样重要的是，通过扫描电子显微镜（SEM）成像来显示银胶体对根管的深层渗透能力，以展示其作为根管填充材料的潜力。
