Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China, and College of Chemistry, Beijing Normal University , Beijing 100875, P. R. China.
Org Lett. 2013 Oct 4;15(19):5020-3. doi: 10.1021/ol4023547. Epub 2013 Sep 16.
A new pH-responsive fluorescent probe has been reported based on protonation-activable resonance charge transfer. In aqueous solution, probe PQ-Lyso exhibits ratiometric detection of pH changes with a large hypsochromic shift of 76 nm and remarkable changes in the fluorescence intensity ratio (R = F(494 nm)/F(570 nm), R/R(0) = 105). Furthermore, PQ-Lyso, which is localized to lysosomes in living cells, can calibrate lysosomal pH using fluorescence ratiometry.
一种新的基于质子激活共振电荷转移的 pH 响应荧光探针已经被报道。在水溶液中，探针 PQ-Lyso 能够进行 pH 值变化的比率检测，其最大的蓝移为 76nm，荧光强度比（R = F(494nm)/F(570nm)，R/R(0) = 105）有显著变化。此外，PQ-Lyso 可以在活细胞的溶酶体中定位，并使用荧光比率法对溶酶体 pH 值进行校准。
