Sun Zhi-Gang, Li Zhen, Yuan Dan-dan, Gao Jun-Fa, Lin Lin, Lin Jie, Zhu Ming-li, Makawana Jigar A, Qian Yong, Zhu Hai-Liang
State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University.
Chem Pharm Bull (Tokyo). 2016;64(1):27-33. doi: 10.1248/cpb.c15-00579. Epub 2015 Oct 30.
We report a novel ratiometric and reversible fluorescent probe for Cd(2+) detection utilizing a 6-(dimethylamino)quinaldine derivative as the fluorophore and a 2-hydrazinopyridine derivative as Cd(2+) chelator. This ratiometric fluorescent probe possesses favorable photophysical properties. It shows a large (55 nm) red-shift from 515 nm to 570 nm in the emission spectrum. Moreover, this probe also exhibits an excellent linear relationship of fluorescence intensity ratio (F570/F515) (R(2)=0.989) vs. Cd(2+) concentration in the range of 0-10 µM at physiological pH, which can serve as a "quantitative detecting" probe for Cd(2+). Utilizing this sensitive and selective probe, we have successfully detected Cd(2+) in living cells.
我们报道了一种新型的用于检测Cd(2+)的比率型可逆荧光探针，该探针利用6-(二甲基氨基)喹哪啶衍生物作为荧光团，2-肼基吡啶衍生物作为Cd(2+)螯合剂。这种比率型荧光探针具有良好的光物理性质。其发射光谱从515nm到570nm有很大的（55nm）红移。此外，该探针在生理pH值下，在0-10µM范围内，荧光强度比（F570/F515）与Cd(2+)浓度也呈现出良好的线性关系（R(2)=0.989），可作为Cd(2+)的“定量检测”探针。利用这种灵敏且选择性好的探针，我们成功地在活细胞中检测到了Cd(2+)。
