Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology , Guangzhou, 510006, China.
J Agric Food Chem. 2013 Oct 16;61(41):9955-60. doi: 10.1021/jf400809r. Epub 2013 Oct 3.
3-Monochloropropane-1,2-diol (3-MCPD) is a well-known food processing contaminant that has been shown to impede the male reproductive function. However, its mechanism of action remains to be elucidated. In this study, the effects of 3-MCPD on progesterone production were investigated using R2C Leydig cells. 3-MCPD caused concentration-dependent inhibition of cell viability at the IC25, IC50, and IC75 levels of 1.027, 1.802, and 3.160 mM, respectively. Single cell gel/comet assay and atomic force microscopy assay showed that 3-MCPD significantly induced early apoptosis. In addition, 3-MCPD significantly reduced progesterone production by reducing the expression of cytochrome P450 side-chain cleavage enzyme, steroidogenic acute regulatory protein, and 3β-hydroxysteroid dehydrogenase in R2C cells. The change in steroidogenic acute regulatory protein expression was highly consistent with progesterone production. Furthermore, the mitochondrial membrane potential and cAMP significantly decreased.
3- 单氯丙二醇（3-MCPD）是一种众所周知的食品加工污染物，已被证明会阻碍男性生殖功能。然而，其作用机制仍有待阐明。在这项研究中，使用 R2C 莱迪希细胞研究了 3-MCPD 对孕酮产生的影响。3-MCPD 在 IC25、IC50 和 IC75 浓度下分别引起浓度依赖性的细胞活力抑制，分别为 1.027、1.802 和 3.160mM。单细胞凝胶/彗星试验和原子力显微镜试验表明，3-MCPD 通过降低 R2C 细胞中细胞色素 P450 侧链裂解酶、类固醇急性调节蛋白和 3β-羟甾脱氢酶的表达，显著诱导早期细胞凋亡。此外，3-MCPD 通过降低细胞色素 P450 侧链裂解酶、类固醇急性调节蛋白和 3β-羟甾脱氢酶的表达显著降低了孕酮的产生。类固醇急性调节蛋白表达的变化与孕酮的产生高度一致。此外，线粒体膜电位和 cAMP 显著降低。
