CSIRO Animal, Food and Health Sciences, Queensland Biosciences Precinct, 306 Carmody Road, St Lucia, QLD 4067, Australia; School of Agriculture and Food Sciences, The University of Queensland, Gatton, QLD 4343, Australia.
Int J Food Microbiol. 2013 Sep 16;166(3):450-7. doi: 10.1016/j.ijfoodmicro.2013.08.014. Epub 2013 Aug 28.
The aim of this study was to examine the antimicrobial potential of three essential oils (EOs: tea tree oil, lemon myrtle oil and Leptospermum oil), five terpenoid compounds (α-bisabolol, α-terpinene, cineole, nerolidol and terpinen-4-ol) and polyphenol against two strains of Campylobacter jejuni (ACM 3393 and the poultry isolate C338), Campylobacter coli and other Gram negative and Gram positive bacteria. Different formulations of neem oil (Azadirachta indica) with these compounds were also tested for synergistic interaction against all organisms. Antimicrobial activity was determined by the use of disc diffusion and broth dilution assays. All EOs tested were found to have strong antimicrobial activity against Campylobacter spp. with inhibitory concentrations in the range 0.001-1% (v/v). Among the single compounds, terpinen-4-ol showed the highest activity against Campylobacter spp. and other reference strains. Based on the antimicrobial activity and potential commerciality of these agents, lemon myrtle oil, α-tops (α-terpineol+cineole+terpinen-4-ol) and terpinen-4-ol were also evaluated using an in vitro fermentation technique to test antimicrobial activity towards C. jejuni in the microbiota from the chicken-caecum. EO compounds (terpinen-4-ol and α-tops) were antimicrobial towards C. jejuni at high doses (0.05%) without altering the fermentation profile. EOs and terpenoid compounds can have strong anti-Campylobacter activity without adversely affecting the fermentation potential of the chicken-caeca microbiota. EOs and their active compounds may have the potential to control C. jejuni colonisation and abundance in poultry.
本研究旨在考察三种精油（茶树油、柠檬桉油和 Leptospermum 油）、五种萜烯化合物（α- 生育醇、α- 松油烯、桉油精、橙花叔醇和萜品-4-醇）和多酚对两种空肠弯曲菌（ACM 3393 和禽源分离株 C338）、大肠弯曲菌和其他革兰氏阴性和革兰氏阳性细菌的抗菌潜力。还测试了含有这些化合物的不同配方的印楝油（Azadirachta indica）与所有生物体的协同相互作用。抗菌活性通过使用圆盘扩散和肉汤稀释测定法来确定。测试的所有精油都对弯曲菌属具有很强的抗菌活性，抑制浓度在 0.001-1%（v/v）范围内。在单一化合物中，萜品-4-醇对弯曲菌属和其他参考菌株表现出最高的活性。基于这些试剂的抗菌活性和潜在的商业性，柠檬桉油、α-萜品（α-松油醇+桉油精+萜品-4-醇）和萜品-4-醇也使用体外发酵技术进行了评估，以测试鸡盲肠微生物群中对空肠弯曲菌的抗菌活性。在高剂量（0.05%）下，精油化合物（萜品-4-醇和 α-萜品）对空肠弯曲菌具有抗菌作用，而不会改变发酵谱。精油和萜烯化合物可以对空肠弯曲菌具有很强的抗菌活性，而不会对鸡盲肠微生物群的发酵潜力产生不利影响。精油及其活性化合物可能具有控制家禽中空肠弯曲菌定植和丰度的潜力。
