Goldstein J, Pollitt N S, Inouye M
Department of Biochemistry, Robert Wood Johnson Medical School, Rutgers University of Medicine and Dentistry of New Jersey, Piscataway 08854-5635.
Proc Natl Acad Sci U S A. 1990 Jan;87(1):283-7. doi: 10.1073/pnas.87.1.283.
When exponentially growing Escherichia coli cell cultures were transferred from 37 degrees C to 10 degrees C or 15 degrees C, the production of a 7.4-kDa cytoplasmic protein (CS7.4) was prominently induced. The rate of CS7.4 production reached 13% of total protein synthesis within 1-1.5 hr after a shift to 10 degrees C and subsequently dropped to a lower basal level. Regulation of CS7.4 expression was very strict, such that synthesis of the protein was undetectable at 37 degrees C. We have cloned the gene encoding this protein and have completed the nucleotide sequence analysis, which revealed that the gene encodes a hydrophilic protein of 70 amino acid residues.
当指数生长的大肠杆菌细胞培养物从37℃转移至10℃或15℃时，一种7.4 kDa的细胞质蛋白（CS7.4）的产生被显著诱导。转移至10℃后1 - 1.5小时内，CS7.4的产生速率达到总蛋白合成的13%，随后降至较低的基础水平。CS7.4表达的调控非常严格，以至于在37℃时无法检测到该蛋白的合成。我们已经克隆了编码此蛋白的基因，并完成了核苷酸序列分析，结果显示该基因编码一个由70个氨基酸残基组成的亲水性蛋白。
